Effects of high temperatures and nitrogen availability on the growth and composition of the marine diatom Chaetoceros pseudocurvisetus

Flanjak, Lana; Vrana Špoljarić, Ivna; Cvitešić Kušan, Ana; Godrijan, Jelena; Novak, Tihana; Penezić, Abra; Gašparović, Blaženka (2022) Effects of high temperatures and nitrogen availability on the growth and composition of the marine diatom Chaetoceros pseudocurvisetus. Journal of Experimental Botany, 73 (12). pp. 4250-4256. ISSN 0022-0957

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Abstract

The assimilation of inorganic nutrients by phytoplankton strongly depends on environmental conditions such as the availability of nitrogen and temperature, especially warming. The acclimation or adaptation of different species to such changes remains poorly understood. Here, we used a multimethod approach to study the viability and physiological and biochemical responses of the marine diatom Chaetoceros pseudocurvisetus to different temperatures (15, 25, and 30 °C) and different N:P ratios. Nitrogen limitation had a greater effect than high temperature on cell growth and reproduction, leading to a marked elongation of setae, decreased phosphorus assimilation, increased lipid accumulation, and decreased protein synthesis. The elongation of setae observed under these conditions may serve to increase the surface area available for the uptake of inorganic and/or organic nitrogen. In contrast, high temperatures (30 °C) had a stronger effect than nitrogen deficiency on cell death, nitrogen assimilation, chlorophyll a accumulation, the cessation of setae formation, and cell lipid remodelling. Significant changes in thylakoid lipids were observed in cells maintained at 30 °C, with increased levels of digalactosyldiacylglycerol and sulfoquinovosyldiacylglycerol. These changes may be explained by the role of galactolipids in thylakoid membrane stabilization during heat stress.

Item Type:	Article
Uncontrolled Keywords:	Global warming; nitrogen stress; diatom Chaetoceros pseudocurvisetus, physiological response; biochemical response; lipid remodeling
Subjects:	NATURAL SCIENCES > Interdisciplinary Natural Sciences NATURAL SCIENCES > Interdisciplinary Natural Sciences > Marine Science
Divisions:	Division for Marine and Enviromental Research
Projects:	Project title Project leader Project code Project type Biokemijski odgovori površinskog sloja oligotrofnog područja Jadranskog mora na atmosfersko taloženje-BiREADI Sanja Frka Milosavljević IP-2018-01-3105 HRZZ Utjecaj okolišnog stresa na pojavnost i međudjelovanje biološki važnih organskih molekula i mikronutrijenata u morskom ekosustavu-AMBIOMERES Blaženka Gašparović IP-2013-11-8607 HRZZ
Depositing User:	Blaženka Gašparović
Date Deposited:	05 Oct 2023 11:02
URI:	http://fulir.irb.hr/id/eprint/8149
DOI:	10.1093/jxb/erac145

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