Effects of combining flow intermittency and exposure to emerging contaminants on the composition and metabolic response of streambed biofilm bacterial communities

Rožman, Marko; Lekunberri, Itziar; Grgić, Ivana; Borrego, Carles; Petrović, Mira (2023) Effects of combining flow intermittency and exposure to emerging contaminants on the composition and metabolic response of streambed biofilm bacterial communities. Science of the Total Environment, 877 . ISSN 1879-1026

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Abstract

Freshwater ecosystems are characterised by the co-occurrence of stressors that simultaneously affect the biota. Among these, flow intermittency and chemical pollution severely impair the diversity and functioning of streambed bacterial communities. Using an artificial streams mesocosmfacility, this study examined how desiccation and pollution caused by emerging contaminants affect the composition of stream biofilm bacterial communities, their metabolic profiles, and interactions with their environment. Through an integrative analysis of the composition of biofilm communities, characterization of their metabolome and composition of the dissolved organic matter, we found strong genotype-tophenotype interconnections. The strongest correlation was found between the composition and metabolismof the bacterial community, both of which were influenced by incubation time and desiccation. Unexpectedly, no effect of the emerging contaminants was observed, which was due to the low concentration of the emerging contaminants and the dominant impact of desiccation. However, biofilm bacterial communities modified the chemical composition of their environment under the effect of pollution. Considering the tentatively identified classes of metabolites, we hypothesised that the biofilm response to desiccation was mainly intracellular while the response to chemical pollution was extracellular. The present study demonstrates that metabolite and dissolved organic matter profiling may be effectively integrated with compositional analysis of stream biofilm communities to yield a more complete picture of changes in response to stressors.

Item Type:	Article
Uncontrolled Keywords:	Contaminants of emerging concern; Streambed biofilms; Desiccation; Global change; Ecosystem services
Subjects:	NATURAL SCIENCES > Chemistry NATURAL SCIENCES > Biology
Divisions:	Division of Physical Chemistry
Projects:	Project title Project leader Project code Project type Višestruki stresori u vodenom okolišu: razumijevanje svojstava stresora i receptora-UNDERSTANDOR Marko Rožman; Sandra Babić; Igor Novak; Ana Previšić; Ivančica Ternjej IP-2018-01-2298 HRZZ Transformation of emerging contaminants in the aquatic environment. Fate of transformation products under multiple stress conditions - TRANSFORMER Marko Rožman H2020 MSCA IF 657425 H2020
Depositing User:	Marko Rožman
Date Deposited:	10 Jan 2025 08:39
URI:	http://fulir.irb.hr/id/eprint/9393
DOI:	10.1016/j.scitotenv.2023.162818

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