Analiza proteinskog profila u stanicama intraepitelnih lezija vrata maternice

Pešut, Ena (2024) Analiza proteinskog profila u stanicama intraepitelnih lezija vrata maternice. Doctoral thesis, Insitiut Ruđer Bošković.

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Abstract

Cervical cancer is the fourth most common cancer in the world by incidence and mortality, and is preceded by precancerous intraepithelial lesions associated with human papillomavirus (HPV) infection. The aim of this study was to analyze the protein profile of cells depending on the stage of the precancerous intraepithelial lesion and the presence of HPV. In this study, the presence of HPV was determined on cervical swabs with precancerous intraepithelial lesions by polymerase chain reaction, and the protein profile was established by mass cytometry and immunocytochemistry. The protein profile of cervical biopsies was determined by immunohistochemistry. The results showed that mass cytometry was suitable for the successful analysis of the protein profile on cervical swabs. The expression of proteins Ck18, Ck8, CD63, CD71, KLF4, LRIG1, E-cadherin, Nanog, p53, Ck17, MCM2, MMP7, and CD29 differed with regard to the severity of the precancerous lesion and the presence of HPV.

Item Type:	Thesis (Doctoral thesis)
Uncontrolled Keywords:	cervical cancer; HPV; CyTOF; SIL; CIN; LBC; protein
Subjects:	NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology
Divisions:	Division of Molecular Medicine
Projects:	Project title Project leader Project code Project type Multifaktorna analiza HPV inficiranih stanica u nastanku raka vrata maternice-MACCD Ivan Sabol; Ivana Erceg Ivkošić; Rajko Fureš; Tomaš Kalina; Branka Molnar Stantić IP-2019-04-3403 HRZZ
Depositing User:	Ena Pešut
Date Deposited:	04 Nov 2024 09:24
URI:	http://fulir.irb.hr/id/eprint/9233

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