Talin and KANK proteins in integrin αVβ5 mediated focal and reticular adhesions in melanoma cell line MDA-MB-435S

Lončarić, Marija (2024) Talin and KANK proteins in integrin αVβ5 mediated focal and reticular adhesions in melanoma cell line MDA-MB-435S. Doctoral thesis, Institut Ruđer Bošković.

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Abstract

Melanoma cell line MDA-MB-435S forms integrin αVβ5 mediated focal (FA) and reticular adhesions (RA). Knockdown of integrin αV increases the sensitivity of MDA-MB-435S cells to the microtubule-targeting antitumor drug paclitaxel and reduces migration. In this work, we showed that the mechanism of these effects is mediated by the binding of talin 2 from FA and KANK2 from the cortical microtubule stabilizing complex. We identified talin 2 as a potential target in tumor therapy. We analyzed RA in MDA-MB-435S cells and discovered KANK2 as a new component of RA.

Item Type:

Thesis (Doctoral thesis)

Uncontrolled Keywords:

Focal Adhesion; Reticular Adhesion; Talin; KANK; Paclitaxel; Melanoma

Subjects:

NATURAL SCIENCES > Biology
BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences

Divisions:

Division of Molecular Biology

Projects: