Genomika i osobitosti ponavljajućih DNA školjkaša iz porodice Ostreidae

Tunjić Cvitanić, Monika (2024) Genomika i osobitosti ponavljajućih DNA školjkaša iz porodice Ostreidae. Doctoral thesis, Sveučilište u Zagrebu.

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Abstract

Repetitive DNA constitutes big, but still uninvestigated genome part. For the oyster Crassostrea gigas it was bioinformatically determined that the repeatome is mainly constituted of satDNAs and mobile elements, and the most abundant satDNA is Cg170. The satellitomes of four closely related species, C. gigas, Crassostrea angulata, Crassostrea virginica and Ostrea edulis, were bioinformatically determined. The most abundant sequences in the centromere of C. gigas are DNA transposons and satDNAs, while in heterochromatin the most abundant are DNA transposons. More than 75% of satellitome of this species showed similarity to Helitrons. Analysis of conserved boxes adjacent to monomers or tandem repeats of satDNAs showed that only one satDNA exists integrated into Helitrons, two have majority of their monomers/tandem repeats as standalone organizational form, and eight satDNAs have equally present monomers/tandem repeats in both forms. All analyzed species have several satDNAs in common, but C. gigas and C. angulata share the highest number of common satDNAs. SatDNAs in C. gigas did not show typical clustering at certain chromosomal positions but new organizational pattern: highly scattered across the genome.

Item Type:	Thesis (Doctoral thesis)
Uncontrolled Keywords:	bivalves; satellitome; repeatome; centromere; heterochromatin
Subjects:	NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology
Divisions:	Division of Molecular Biology
Projects:	Project title Project leader Project code Project type Centromerna genomika beskralježnjaka-CENGEN Miroslav Plohl IP-2014-09-3183 HRZZ Rasvjetljavanje evolucije satelitnih DNA visokoprotočnim analizama satelitoma srodnih vrsta-EvoSat Brankica Mravinac; Tena Gržan; Juan J. Pasantes; Daniel Garcia Souto IP-2019-04-5522 HRZZ
Depositing User:	Monika Tunjić Cvitanić
Date Deposited:	29 Apr 2024 12:10
URI:	http://fulir.irb.hr/id/eprint/8798

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