Head and neck tumor cells exhibit altered proliferation upon overexpression of nm23 genes

Herak Bosnar, Maja; Dubravčić, Klara; Bago, Ružica; Pavelić, Jasminka (2008) Head and neck tumor cells exhibit altered proliferation upon overexpression of nm23 genes. Croatica Chemica Acta, 81 (1). pp. 183-189. ISSN 0011-1643

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Abstract

nm23 was identified as a metastasis suppressor gene but is also appointed to a number of other biological functions. The goal of this study was to reveal the influence of ectopic expression of nm23-H1 and mn23-H2 on proliferation properties of head and neck tumor cells. The proliferation rate of transfected cells was evaluated using EGFP reporter system and flow cytometry. HEp-2 and CAL 33 cells transiently transfected with nm23 cDNA containing constructs exhibited enhanced proliferation. CAL 27 cells constitutively expressing GFP-Nm23-H2 protein, exhibited intense proliferation the first day after seeding, while the GFP-Nm23-H1 expressing clone started to proliferate after one-day lag period. The results on transiently transfected HEp-2 and CAL 33 cells generally confirmed previous findings connecting nm23 expression with altered proliferation of head and neck tumors. We speculate that the effects observed on stably transfected CAL 27 clones are due to their different attachment properties.

Item Type:	Article
Uncontrolled Keywords:	nm23; head and neck tumor cell lines; flow cytometry; green fluorescent protein; nm23/nucleoside diphosphate kinase; human breast-cancer; squamous-cell; protein expression; flow-cytometry; metastasis; carcinomas; suppressor; disease; progression
Subjects:	NATURAL SCIENCES > Chemistry
Divisions:	Division of Molecular Medicine
Projects:	Project title Project leader Project code Project type Uloga mreže proteina p53/p73 u sarkomima mekih tkiva čovjeka [173221] Neda Slade 098-0982464-2391 MZOS Molekularno profiliranje metastazirajućeg tumora dojke [9136] Dragan Dekaris 101-0982464-2277 MZOS Gensko liječenje tumora djelovanjem na molekule imunološkog sustava [79061] Jasminka Pavelić 098-0982464-2394 MZOS Uloga gena nm23 u karcinomu pločastog epitela usne šupljine [202030] Maja Herak-Bosnar 098-0982464-2513 MZOS
Depositing User:	Maja Herak Bosnar
Date Deposited:	25 Oct 2013 12:17
URI:	http://fulir.irb.hr/id/eprint/842

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