Expression of Oxidative Stress and Inflammation-Related Genes in Nasal Mucosa and Nasal Polyps from Patients with Chronic Rhinosinusitis

Mihalj, Hrvoje; Butković, Josip; Tokić, Stana; Štefanić, Mario; Kizivat, Tomislav; Bujak, Maro; Baus Lončar, Mirela; Mihalj, Martina (2022) Expression of Oxidative Stress and Inflammation-Related Genes in Nasal Mucosa and Nasal Polyps from Patients with Chronic Rhinosinusitis. International Journal of Molecular Sciences, 23 (10). ISSN 1422-0067

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Abstract

Chronic rhinosinusitis (CRS) is a prevalent, multifaceted inflammatory condition affecting the nasal cavity and the paranasal sinuses, frequently accompanied by formation of nasal polyps (CRSwNP). This apparently uniform clinical entity is preceded by heterogeneous changes in cellular and molecular patterns, suggesting the presence of multiple CRS endotypes and a diverse etiology. Alterations of the upper airway innate defense mechanisms, including antimicrobial and antioxidant capacity, have been implicated in CRSwNP etiology. The aim of this study was to investigate mRNA expression patterns of antioxidative enzymes, including superoxide dismutase (SOD) and peroxiredoxin-2 (PRDX2), and innate immune system defense players, namely the bactericidal/permeability-increasing fold-containing family A, member 1 (BPIFA1) and PACAP family members, particularly adenylate-cyclase-activating polypeptide receptor 1 (ADCYAP1) in nasal mucosa and nasal polyps from CRSwNP patients. Additional stratification based on age, sex, allergic comorbidity, and disease severity was applied. The results showed that ADCYAP1, BPIFA1, and PRDX2 transcripts are differentially expressed in nasal mucosa and scale with radiologically assessed disease severity in CRSwNP patients. Sinonasal transcriptome is not associated with age, sex, and smoking in CRSwNP. Surgical and postoperative corticosteroid (CS) therapy improves endoscopic appearance of the mucosa, but variably reverses target gene expression patterns in the nasal cavity of CRSwNP patients. Transcriptional cross-correlations analysis revealed an increased level of connectedness among differentially expressed genes under inflammatory conditions and restoration of basic network following CS treatment. Although results of the present study imply a possible engagement of ADCYAP1 and BPIFA1 as biomarkers for CRSwNP, a more profound study taking into account disease severity and CRSwNP endotypes prior to the treatment would provide additional information on their sensitivity.

Item Type:	Article
Uncontrolled Keywords:	chronic rhinosinusitis with nasal polyps; superoxide dismutase; peroxiredoxin-2; adenylate-cyclase-activating polypeptide receptor 1; bactericidal/permeability-increasing fold-containing family A; member 1
Subjects:	NATURAL SCIENCES > Biology BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences BIOMEDICINE AND HEALTHCARE > Clinical Medical Sciences
Divisions:	Division of Materials Chemistry Division of Molecular Medicine
Projects:	Project title Project leader Project code Project type Utjecaj mikrobioma na fenotip i funkcionalni status gama delta T limfocita u sinonazalnoj sluznici oboljelih od kroničnog rinosinuitisa s nosnom polipozom- Hrvoje Mihalj VIF 2022 NadSve Imunotranskriptom perifernih MR1+TCRVα7.2+ limfocita u vulgarnoj psorijazi: RNASeq analiza- Stana Tokić MEFOS-IP15-2022 MZO Ustanova
Depositing User:	Diana Mikoč Radešić
Date Deposited:	25 Jan 2024 12:29
URI:	http://fulir.irb.hr/id/eprint/8395
DOI:	10.3390/ijms23105521

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