Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus

Ljoljić Bilić, Vanja; Gašić, Uroš M.; Milojković-Opsenica, Dušanka; Rimac, Hrvoje; Vuković Rodriguez, Jadranka; Vlainić, Josipa; Brlek-Gorski, Diana; Kosalec, Ivan (2022) Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus. Antibiotics, 11 (4). p. 492. ISSN 2079-6382

PDF - Published Version - article Available under License Creative Commons Attribution. Download (1MB)

Abstract

Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains—both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental design consisted of general methods (the serial microdilution broth assay and the agar well diffusion assay), as well as observing bactericidal/bacteriostatic activity through time (the “time-kill” assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial hemolysis. Observed antibacterial activity from above-described methods led to further activity-guided fractionation of water and methanol extracts using bioautography coupled with UHPLC-LTQ OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl-shikimic acid was identified as the most abundant compound. These results point to a direct connection between galloyl-shikimic acid and the observed E. cicutarium antibacterial activity, and open several new research approaches for future investigation.

Item Type:	Article
Uncontrolled Keywords:	Erodium cicutarium; MRSA; biofilm; bioautography; fractionation; anti-hemolytic; phenolic composition; galloyl-shikimic acid
Subjects:	BIOMEDICINE AND HEALTHCARE > Pharmacy
Divisions:	Division of Molecular Medicine
Depositing User:	Lorena Palameta
Date Deposited:	13 Dec 2023 13:22
URI:	http://fulir.irb.hr/id/eprint/8304
DOI:	10.3390/antibiotics11040492

Actions (login required)

View Item