Arab-1, a GDSL Lipase from the Model Plant, Arabidopsis thaliana (L.) Heynh.

Mikleušević, Goran; Salopek Sondi, Branka; Luić, Marija (2009) Arab-1, a GDSL Lipase from the Model Plant, Arabidopsis thaliana (L.) Heynh.. Croatica Chemica Acta, 82 (2). pp. 439-447. ISSN 0011-1643

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Abstract

Bioinformatics analysis of the protein Arab-1 from model plant Arabidopsis thaliana (L.) Heynh. was performed at the level of primary, secondary and tertiary structure. Results suggest that Arab-1 belongs to the important but structurally very poorely characterised GDSL (SGNH) family of hydrolases. The amino acids Ser42, Gly118, Asn180, Asp346 and His349 are predicted as the active center residues. According to phylogenetic analysis, Arab-1 is related to Brassica napus lipases. Attempts to produce recombinant Arab-1, using pET and pRSET expression systems, and several strains of Escherichia coli cells are described; the procedures were found to be inadequate for the production of amounts of protein sufficient for crystallisation. The problems observed in protein production are discussed in view of bioinformatics analyses and calculated physico-chemical parameters.

Item Type:	Article
Uncontrolled Keywords:	SGNH (GDSL) hydrolase; lipase; Arabidopsis thaliana; heterologous protein expression; bioinformatics analysis; phylogenetic analysis; secondary structure prediction; protein; sequence; family; alignment; expression; resource; matrices; database; enzymes
Subjects:	NATURAL SCIENCES > Chemistry
Divisions:	Division of Molecular Biology Division of Physical Chemistry
Projects:	Project title Project leader Project code Project type Molekularna regulacija biljnog razvitka [200002] Branka Salopek-Sondi 098-0982913-2829 MZOS Protein-ligand međudjelovanja na atomnoj razini [77430] Marija Luić 098-1191344-2943 MZOS
Depositing User:	Marija Luić
Date Deposited:	18 Oct 2013 09:10
URI:	http://fulir.irb.hr/id/eprint/814

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