Novosel, Nives
(2022)
Ponašanje i površinska svojstva mikroalga kao pokazatelji stresa u vodenim ekosustavima.
Doctoral thesis, Sveučilište u Zagrebu, Prirodoslovno matematički fakultet.
Abstract
The objective of the PhD thesis was to relate the cellular response of microalgae in terms of behavior and surface properties in relation to anthropogenic stressors (heavy metals) and abiotic stressors (temperature, salinity) to better understand cellular survival and adaptation strategies in aquatic ecosystems under climate change. The study was conducted with three monocultures of microalgae under laboratory conditions using the electrochemical method and the software package ICY with statistical processing. The results showed that the adaptive response of microalgae depends on the studied species and its structural characteristics of the cell barrier, as well as on the type of stressor. When the cells are exposed to toxic cadmium concentration and the temperature and salinity decrease, the flagellate species become poorly motile, cells become softer, change the degree of hydrophobicity and physiological activity, and may enter the dormant stage. Diatom Cylindrotheca closterium has been shown to be the most tolerant to changes in both abiotic stressors compared to green algae Dunaliella tertiolecta and Tetraselmis suecica. Taking into consideration that the behavior and surface properties of microalgae show a measurable difference from control conditions, they can be considered indicators of stress in aquatic ecosystems.
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