Topić Popović, Natalija; Kazazić, Snježana; Čož- Rakovac, Rozelinda (2022) MALDI-TOF MS profiling of piscine Acinetobacter spp. from wastewater-related waters. Veterinarski glasnik, 00 . pp. 1-8. ISSN 0350-2457
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Abstract
Acinetobacter species are common inhabitants of freshwater and marine ecosystems with a capacity to induce disease in affected fish. To facilitate their rapid and reliable identification, matrix- assisted laser desorption/ionization time-of- flight mass spectrometry (MALDI-TOF MS), was applied to Acinetobacter from fish. The aims of the study were, thus, to identify and profile the Acinetobacter species from tissues of fish living in a vulnerable environment impacted by wastewaters, and to assess the potential of MALDI- TOF MS as a method for discriminating these acinetobacters. Fish were sampled from waterways impacted by the activity of a wastewater treatment plant. Samples of gills, spleen, kidney and liver were streaked onto general-purpose media to purity. The profiling and identification of acinetobacters was conducted with MALDITOF MS, with the samples prepared by ethanol/formic acid extraction. The identified acinetobacters were retrieved from gills (68.96 %), kidney (13.79 %), liver (10.34 %), and spleen (6.89 %). The Acinetobacter species isolated from all tested fish tissues were A. johnsonii (79.31 %), A. pittii (10.34 %), A. tandoii (3.44 %), A. guilouiae (3.44 %), and A. gerneri (3.44 %). Highly probable and probable species identifications were obtained for 48.27 % of all acinetobacters tested, indicating fully reliable identification. MALDI‐TOF MS gave excellent identification and profiling results for piscine Acinetobacter species from the wastewater-affected waterways. It is a recommendable technique for future Acinetobacter species discrimination, as accurate and rapid identification of these bacteria is critical in both environmental pollution management and human/veterinary clinical diagnostics.
| Item Type: | Article | ||||||||
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| Uncontrolled Keywords: | Acinetobacter, fish, MALDI-TOF MS, wastewater | ||||||||
| Subjects: | NATURAL SCIENCES > Biology | ||||||||
| Divisions: | Division of Materials Chemistry Division of Physical Chemistry |
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| Depositing User: | Natalija Topić Popović | ||||||||
| Date Deposited: | 11 Jul 2022 07:27 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/7461 | ||||||||
| DOI: | 10.2298/VETGL211201005T |
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