Detection of Renibacterium salmoninarum in tissue of brook trout (Salvelinus fontinalis) by nested RT-PCR

Vardić Smrzlić, Irena; Kapetanović, Damir; Valić, Damir; Kurtović, Božidar; Teskeredžić, Zlatica; Teskeredžić, Emin (2007) Detection of Renibacterium salmoninarum in tissue of brook trout (Salvelinus fontinalis) by nested RT-PCR. Ribarstvo : znanstveno-stručni časopis za ribarstvo, 65 (1). pp. 15-24. ISSN 1330-061X

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Abstract

Pathogenic bacterium Renibacterium salmoninarum causes kidney disease with high mortality rate and considerable economic losses in salmonid farming. Thus, application of fast and sensitive method for R. salmoninarum diagnosis is of great importance. This paper describes detection of R. salmoninarum in brook trout tissue with gross clinical signs of disease by nested RT-PCR. Determination of partial sequence of bacterial msa gene was done prior to comparison with similar sequences from different R. salmoninarum isolates. Nested RT-PCR proved to be a rapid and valuable diagnostic tool for R. salmoninarum detection, and sequence analysis confirmed previously reported genetic uniformity of this bacteria.

Item Type:	Article
Uncontrolled Keywords:	Renibacterium salmoninarum ; nested RT-PCR ; msa gene sequence ; brook trout
Subjects:	BIOMEDICINE AND HEALTHCARE > Veterinary Medicine
Divisions:	Division for Marine and Enviromental Research
Projects:	Project title Project leader Project code Project type Patologija organizama iz voda u odnosu na zagađivala i akvakulturu Kapetanović, Damir 098-0982934-2752 MZOS
Depositing User:	Damir Kapetanović
Date Deposited:	09 Feb 2022 12:49
URI:	http://fulir.irb.hr/id/eprint/6983

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