Supercritical Carbon Dioxide Extraction of Four Medicinal Mediterranean Plants: Investigation of Chemical Composition and Antioxidant Activity

Čižmek, Lara; Bavcon Kralj, Mojca; Čož-Rakovac, Rozelindra; Mazur, Dmitrii; Ul’yanovskii, Nikolay; Likon, Marko; Trebše, Polonca (2021) Supercritical Carbon Dioxide Extraction of Four Medicinal Mediterranean Plants: Investigation of Chemical Composition and Antioxidant Activity. Molecules, 26 (18). ISSN 1420-3049

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Abstract

With everyday advances in the field of pharmaceuticals, medicinal plants have high priority regarding the introduction of novel synthetic compounds by the usage of environmentally friendly extraction technologies. Herein, a supercritical CO2 extraction method was implemented in the analysis of four plants (chamomile, St. John’s wort, yarrow, and curry plant) after which the non-targeted analysis of the chemical composition, phenolic content, and antioxidant activity was evaluated. The extraction yield was the highest for the chamomile (5%), while moderate yields were obtained for the other three plants. The chemical composition analyzed by gas chromatographyhigh-resolution mass spectrometry (GC-HRMS) and liquid chromatography- high-resolution mass spectrometry (LC-HRMS) demonstrated extraction of diverse compounds including terpenes and terpenoids, fatty acids, flavonoids and coumarins, functionalized phytosterols, and polyphenols. Voltammetry of microfilm immobilized on a glassy carbon electrode using square-wave voltammetry (SWV) was applied in the analysis of extracts. It was found that antioxidant activity obtained by SWV correlates well to 1, 1-diphenyl-2-picrylhidrazine (DPPH) radical assay (R2 = 0.818) and ferric reducing antioxidant power (FRAP) assay (R2 = 0.640), but not to the total phenolic content (R2 = 0.092). Effective results were obtained in terms of activity showing the potential usage of supercritical CO2 extraction to acquire bioactive compounds of interest.

Item Type:	Article
Uncontrolled Keywords:	green solvents ; chamomile ; yarrow ; St. John’s wort ; curry plant ; square-wave voltammetry ; chromatography
Subjects:	NATURAL SCIENCES > Chemistry BIOTECHNICAL SCIENCES > Biotechnology
Divisions:	Division of Materials Chemistry
Depositing User:	Lara Čižmek
Date Deposited:	31 Dec 2021 10:27
URI:	http://fulir.irb.hr/id/eprint/6862
DOI:	10.3390/molecules26185697

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