Ganglioside Composition Distinguishes Anaplastic Ganglioglioma Tumor Tissue from Peritumoral Brain Tissue: Complementary Mass Spectrometry and Thin-Layer Chromatography Evidence

Fabris, Dragana; Karmelić, Ivana; Muharemović, Hasan; Sajko, Tomislav; Jurilj, Mia; Potočki, Slavica; Novak, Ruđer; Vukelić, Željka (2021) Ganglioside Composition Distinguishes Anaplastic Ganglioglioma Tumor Tissue from Peritumoral Brain Tissue: Complementary Mass Spectrometry and Thin-Layer Chromatography Evidence. International journal of molecular sciences, 22 (16). ISSN 1422-0067

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Abstract

Gangliosides serve as antitumor therapy targets and aberrations in their composition strongly correlate with tumor growth and invasiveness. Anaplastic ganglioglioma is a rare, poorly characterized, malignant neuronal–glial tumor type. We present the first comparative characterization of ganglioside composition in anaplastic ganglioglioma vs. peritumoral and healthy brain tissues by combining mass spectrometry and thin-layer chromatography. Anaplastic ganglioglioma ganglioside composition was highly distinguishable from both peritumoral and healthy tissue despite having five to six times lower total content. Ten out of twelve MS-identified ganglioside classes, defined by unique glycan residues, were represented by a large number and considerable abundance of individual species with different fatty acid residues (C16–C24) in ceramide portions. The major structurally identified class was tumor-associated GD3 (>50%) with 11 species ; GD3 (d18:1/24:0) being the most abundant. The dominant sphingoid base residue in ganglioside ceramides was sphingosine (d18:1), followed by eicosasphingosine (d20:1). The peritumoral tissue ganglioside composition was estimated as normal. Specific ganglioside composition and large variability of ganglioside ceramide structures determined in anaplastic ganglioglioma demonstrate realistic ganglioside expression patterns and correspond to the profile of high-grade malignancy brain tumors.

Item Type:	Article
Uncontrolled Keywords:	ceramides ; ganglioglioma ; gangliosides ; mass spectrometry ; sphingolipids
Subjects:	BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences
Divisions:	Division of Physical Chemistry
Projects:	Project title Project leader Project code Project type Uspostava inovativne kategorije biomarkera tumora i krvnih ugrušaka mozga pomoću analize sfingolipidomskog profila Fabris, Dragana 13127 DGS Strukturno-funkcionalna glikolipidomika moždanog razvitka i maligne alteracije Vukelić, Željka 108-1081870-2415 MZOS
Depositing User:	Hasan Muharemović
Date Deposited:	25 Aug 2021 09:17
URI:	http://fulir.irb.hr/id/eprint/6542
DOI:	10.3390/ijms22168844

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