miR-7 AND miR-34a sequence cloning and expression in a1235 glioblastoma cell line

Kolić, Dora; Horvat, Luka; Šetinc, Maja; Antica, Mariastefania; Matulić, Maja (2020) miR-7 AND miR-34a sequence cloning and expression in a1235 glioblastoma cell line. Molecular and experimental biology in medicine, 3 (2). pp. 31-38. ISSN 2584-671X

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Abstract

miRNAs are small non-coding RNAs which have an important role in signalling circuits regulating different cell processes. miR-7 and miR-34a are known as tumour suppressors, and both of them can interfere with cell proliferation, differentiation, apoptosis and migration. We constructed plasmids containing pri-miRNA sequences for these two miRNAs and introduced them into the A1235 glioblastoma cell line. Clones containing increased expression of processed miR-7 and miR-34a were obtained. The proliferation and sensitivity to alkylation agent of transfected cells were similar to those of control cells. Our results indicate that an increase in miR-7 and miR34 expression alone in A1235 glioblastoma cells is not sufficient to change their proliferation or sensitivity to the influence of alkylating agents.

Item Type:	Article
Uncontrolled Keywords:	miR - 7 ; miR - 34a ; expression ; glioblastoma cell line
Subjects:	NATURAL SCIENCES > Biology
Divisions:	Division of Molecular Biology
Projects:	Project title Project leader Project code Project type Notch u diferencijaciji hematopoetskih stanica matica i u razvoju leukemija Antica, Mariastefania IP-2013-11-7140 HRZZ
Depositing User:	Mariastefania Antica
Date Deposited:	19 Jan 2021 08:44
URI:	http://fulir.irb.hr/id/eprint/6173
DOI:	10.33602/mebm.3.2.4

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