Fluorescent cyanine-guanidiniocarbonyl-pyrrole conjugate with pH-dependent DNA/RNA recognition and DPP III fluorescent labelling and inhibition properties

Šmidlehner, Tamara; Karačić, Zrinka; Tomić, Sanja; Schmuck, Carsten; Piantanida, Ivo (2018) Fluorescent cyanine-guanidiniocarbonyl-pyrrole conjugate with pH-dependent DNA/RNA recognition and DPP III fluorescent labelling and inhibition properties. Monatshefte für Chemie, 149 (7). pp. 1307-1313. ISSN 0026-9247

Preview

PDF - Published Version - article Available under License Creative Commons Attribution. Download (1MB) | Preview

Abstract

Here designed and prepared cyanine - guanidinocarbonyl-pyrrole conjugate (Cy-GCP), intrinsically non-fluorescent, revealed fluorescent switch-on recognition of various secondary structures of ds-DNA and ds-RNA. Moreover, at the same submicromolar concentrations, DNA/RNA recognition was observed by selective induced (I)CD pattern in the visible range. Moreover, preliminary results showed that Cy-GCP strongly interacted with DPP III enzyme, switching-on the fluorescence upon binding and inhibiting enzyme action with efficiency comparable to the best-known inhibitor tynorphin.

Item Type:	Article
Uncontrolled Keywords:	DNA/RNA recognition ; Circular dichroism ; Fluorescence ; DPP III enzyme binding ; DPP III inhibition
Subjects:	NATURAL SCIENCES > Chemistry
Divisions:	Division of Organic Chemistry and Biochemistry
Projects:	Project title Project leader Project code Project type Višenamjensko očitavanje DNA/RNA sekundarne strukture molekularnim kemijskim senzorima (DNA/RNA-MolSense) Piantanida, Ivo HRZZ-IP-2013-11-1477 HRZZ
Depositing User:	Zrinka Karačić
Date Deposited:	28 Jul 2023 07:39
URI:	http://fulir.irb.hr/id/eprint/5824
DOI:	10.1007/s00706-018-2192-0

Actions (login required)

View Item