CD36 expression in peripheral blood mononuclear cells reflects the onset of atherosclerosis

Yazgan, Burak; Sozen, Erdi; Karademir, Betul; Ustunsoy, Seyfettin; Ince, Umit; Žarković, Neven; Ozer, Nesrin Kartal (2018) CD36 expression in peripheral blood mononuclear cells reflects the onset of atherosclerosis. BioFactors, 44 (6). pp. 588-596. ISSN 0951-6433

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Abstract

Together with complex genetic and environmental factors, increased serum cholesterol and ox-LDL levels are considered as major triggering factors of atherosclerosis. Mononuclear cell infiltration to the arterial wall and uptake of ox-LDL, which is facilitated by CD36 receptor through an uncontrolled manner, play a key role in foam cell formation followed by atherogenesis development. The aim of this study was to analyze if CD36 expression in peripheral blood mononuclear cells reflect its aortic tissue level in hypercholesterolemia. In this study, CD36 protein expression was evaluated in aortic specimens of cholesterol or cholesterol plus Vitamin E treated animals in relation to the immunohistochemical analyses for the HNEprotein adducts, as well as for smooth muscle actin and vimentin. The CD36 mRNA expression was determined by RTPCR in PBMC of hypercholesterolemic rabbits and hypercholesterolemic versus normocholesterolemic individuals. Immunohistochemistry findings revealed that smooth muscle actin, smooth muscle vimentin, HNE-protein conjugates, and CD36 protein expressions were significantly increased in aorta of hypercholesterolemic group where foam cells were present. High cholesterol diet significantly induced CD36 mRNA expression in both rabbit aorta and PBMCs, while positive correlation between aortic and PBMC CD36 expression has been found. In addition, consistent with the rabbit model, CD36 mRNA expression levels in human PBMCs were significantly higher in hypercholesterolemic patients than in normocholesterolemic individuals. Taken together, these results demonstrate that the CD36 mRNA levels of PBMCs could reflect the CD36 mRNA levels in aorta and could be used as a biomarker for diagnosis of atherosclerotic burden.

Item Type:	Article
Additional Information:	This work was supported by COST B35 Action, TUBITAK (106S121), and Marmara University Research Fund SAG-C-YLP-050608-0125. BY, ES, and NKO designed research, analyzed data and wrote the article; BY, ES, SU, and BK conducted reseach; UI conducted microscop
Uncontrolled Keywords:	hypercholesterolemia ; atherosclerosis ; PBMC ; CD36 receptor
Subjects:	BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences
Divisions:	Division of Molecular Medicine
Depositing User:	Tea Vuković
Date Deposited:	04 May 2020 11:03
URI:	http://fulir.irb.hr/id/eprint/5676
DOI:	10.1002/biof.1372

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