The effects of diet supplemented with Lactobacillus rhamnosus on tissue parameters of rainbow trout, Oncorhynchus mykiss (Walbaum)

Topić Popović, Natalija; Strunjak-Perović, Ivančica; Sauerborn Klobučar, Roberta; Barišić, Josip; Jadan, Margita; Kazazić, Snježana; Kesner-Koren, Inga; Prevendar Crnić, Andreja; Šuran, Jelena; Beer Ljubić, Blanka; Matijatko, Vesna; Rozelinda, Čož-Rakovac (2017) The effects of diet supplemented with Lactobacillus rhamnosus on tissue parameters of rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Research, 48 (5). pp. 2388-2401. ISSN 1355-557X

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/are.130...

Abstract

This study was carried out in order to establish the effects of a 6-week treatment with the diet supplemented with L. rhamnosus in concentrations of 10*7 CFU g-1 (G1 group) and 10*8 CFU g-1 (G2 group) on the condition expressed by condition factors (Fulton’s, Clark’s and B), intestinal microbiology, haematological, histological, and selected antioxidative parameters of rainbow trout. A significantly higher condition factors was found in G1 group indicating that higher concentration of probiotic (10*8 CFU g-1) did not result in the better condition. Cholesterol and urea levels were significantly higher in both G1 and G2 groups, albumin in G1 and creatinine in G2 group with respect to control. A significantly higher liver TBARS level was observed in G2 group. The feeding with supplemented probiont apparently changed the resident microbiota. Three weeks after withdrawal of the supplemented feed, the microflora mostly reverted to the control composition, although L. rhamnosus in fecal matter of fish remained inherent. The epithelial structure of the proximal and distal intestine revealed the increased absorptive area in both treated groups, as well as the increase of the mucin-secreting goblet cells. The L. rhamnosus treated groups demonstrated the capacity for the augmentation of the innate host defense.

Item Type:

Article

Additional Information:

This study was supported by a grant from the Croatian Ministry of Agriculture (VIP Project ur.br. 03-8473/1-2012; 2012-2014). The authors thank the staff of the Croatian Center for Indigenous Species of Fish and Crayfish in Karstic Waters for taking excellent care of the fish in trial.

Uncontrolled Keywords:

L. rhamnosus ; trout ; blood ; tissue responses

Subjects:

BIOMEDICINE AND HEALTHCARE > Veterinary Medicine

Divisions:

Division of Materials Chemistry
Division of Physical Chemistry

Projects: