Jaganjac, Morana; Milković, Lidija; Čipak Gašparović, Ana; Mozetič, Miran; Recek, Nina; Žarković, Neven; Vesel, Alenka
(2012)
Cell adhesion on hydrophobic polymer surfaces.
Materiali in Tehnologije, 46
(1).
pp. 53-56.
ISSN 1580-2949
Abstract
Adhesion of human osteosarcoma (HOS) cells on hydrophobic polymer surface was studied. Surface of polymer polystyrene (PS) was made hydrophobic by treatment in plasma created in tetrafluoromethane gas (CF4). The PS samples were exposed for 30 s to CF4 plasma created by RF generator powered at 200 W. This treatment time allowed for optimal polymer surface functionalization with fluorine functional groups. This caused an increase of surface hydrophobicity from initial 85° to about 110° as measured by water contact angle. The HOS cells were deposited on untreated and plasma treated samples and incubated for 1, 2 and 6 days. Both untreated and plasma treated samples were tested for biocompatibility by two different methods: optical micrographs were used to study the cell morphology and MTT test was used to study the cell viability. The results showed better adhesion of cells on plasma treated samples with more hydrophobic surface in comparison to the untreated sample. MTT test revealed about 1.6-times higher activity of cell enzymes after 6-day incubation for plasma treated sample. Optical micrographs have shown that both untreated and fluorine-plasma treated polymer surfaces are not optimal for cell proliferation, since cells need about 2 days to adapt to the surface. After this adaptation time cells start to proliferate on the polymer surface, especially, on that one treated in plasma.
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