Microbial anchoring systems for cell-surface display of lipolytic enzymes

Bielen, Ana; Teparić, Renata; Vujaklija, Dušica; Mrša, Vladimir (2014) Microbial anchoring systems for cell-surface display of lipolytic enzymes. Food Technology and Biotechnology, 52 (1). pp. 16-34. ISSN 1330-9862

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Abstract

Studies of microbial cell envelopes and particularly cell surface proteins and mechanisms of their localization brought about new biotechnological applications of gained knowledge in surface display of homologous and heterologous proteins. By fusing surface proteins, or their anchoring domains with different proteins of interest their so called genetic immobilization is achieved. Hybrid proteins are engineered in a way that they are expressed in the host cells, secreted to the cell surface and incorporated into the wall/envelope moiety. In this way laborious and often detrimental procedure of chemical immobilization of the protein is avoided by letting the cells do the whole procedure. Both bacterial and yeast cells have been used for this purpose and a number of potential biotechnological applications of surface displayed proteins have been reported. Among the most frequently used passenger proteins are lipolytic enzymes, due to their great technological significance and numerous important applications. In this review our current knowledge on mechanisms and molecular systems for surface display of lipolytic enzymes on bacterial and yeast cell surfaces is summarized.

Item Type:	Article
Uncontrolled Keywords:	surface display; genetic immobilization; lipolytic enzymes; bacterial envelope; yeast cell wall
Subjects:	BIOTECHNICAL SCIENCES > Biotechnology
Divisions:	Division of Molecular Biology
Projects:	Project title Project leader Project code Project type Temeljna molekularno-biološka istraživanja streptomiceta Dušica Vujaklija 098-0982913-2877 MZOS Molekularni mehanizmi ugradnje proteina u staničnu stijenku kvasca Vladimir Mrša 058-0580477-2240 MZOS
Depositing User:	Dušica Vujaklija
Date Deposited:	21 Jul 2016 10:56
URI:	http://fulir.irb.hr/id/eprint/2976

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