Advantages and disadvantages of voltammetric method in studying cadmium-metallothionein interactions

Erk, Marijana; Raspor, Biserka (2000) Advantages and disadvantages of voltammetric method in studying cadmium-metallothionein interactions. Cellular and molecular biology, 46 (2). pp. 269-281. ISSN 0145-5680

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Abstract

A sensitive and chemical species-selective technique of differential pulse anodic stripping voltammetry (DPASV) was applied in studying the cadmium-metallothionein (Cd-MT) interaction. The amperometric titrations of the purified MT20 and MT10 fractions, isolated by verified biochemical procedures from the digestive gland of cadmium-exposed mussels Mytilus galloprovincialis, with Cd^2+ ions were performed in the buffered sodium chloride solution of 0.59 M ionic strenght, pH=7.9 and 25°C. Applying the DPASV method at various cadmium to metallothionein ratio several groups of chemical species were recorded. The data on the available ligand concentration to complex cadmium ions (C_L), the apparent concentration stability constants (K^,) of the respective complexes and the reliability of the determined complexing parameters are discussed. In quantifying the Cd-MT interaction the interference of dithiotreitol (DTT), which is used as the reducing agent in isolation and purification of MTs, is documented.

Item Type:	Article
Uncontrolled Keywords:	Metallothionein; Isoforms; Dithiotreitol; Cadmium binding; Mytilus galloprovincialis; Voltammetry
Subjects:	NATURAL SCIENCES > Chemistry NATURAL SCIENCES > Interdisciplinary Natural Sciences NATURAL SCIENCES > Interdisciplinary Natural Sciences > Environmental Science
Divisions:	Division for Marine and Enviromental Research
Depositing User:	Marijana Erk
Date Deposited:	10 Jul 2015 08:56
URI:	http://fulir.irb.hr/id/eprint/2064

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