Spectrophotometric analysis of flavonoid-DNA interactions and DNA damaging/protecting and cytotoxic potential of flavonoids in human peripheral blood lymphocytes

Rusak, Gordana; Piantanida, Ivo; Mašić, Lozika; Kapuralin, Katarina; Durgo, Ksenija; Kopjar, Nevenka (2010) Spectrophotometric analysis of flavonoid-DNA interactions and DNA damaging/protecting and cytotoxic potential of flavonoids in human peripheral blood lymphocytes. Chemico-Biological Interactions, 188 (1). pp. 181-189. ISSN 0009-2797

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Abstract

The ability of luteolin, kaempferol and apigenin to bind to calf thymus (ct)-DNA, mode of action and stability of flavonoids in buffer were investigated. Spectrophotometric analysis revealed a rapid degradation of apigenin in an aqueous medium, while kaempferol and luteolin were stable for 24 h upon dissolution in water. Spectrophotometric study of the interactions of kaempferol and luteolin with calf thymus DNA suggests classic intercalation as their dominant binding mode to DNA. Cytotoxicity/genotoxicity and cytoprotective/genoprotective effects of flavonoids in non-stressed and hydrogen peroxide stressed human peripheral lymphocytes were investigated using the fluorescent dye exclusion method and alkaline comet assay. Flavonoids revealed significant genoprotective effects in hydrogen peroxide stressed cells and in cells submitted to longer incubation in the cell culture medium. Luteolin, followed by apigenin and kaempferol, was shown to be the most effective in protecting DNA from oxidative damage induced by hydrogen peroxide. However, the investigated flavonoids also induced DNA damage, indicating their prooxidative capacity. The balance between the protection of DNA from oxidative damage and prooxidative effects was strongly dependent on flavonoid concentration and the incubation period.

Item Type:	Article
Uncontrolled Keywords:	apigenin; kaempferol; luteolin; DNA; lymphocyte; cytoprotective/genoprotective effects
Subjects:	NATURAL SCIENCES > Chemistry > Organic Chemistry BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences
Divisions:	Division of Organic Chemistry and Biochemistry
Projects:	Project title Project leader Project code Project type Genotoksičnost kemijskih i fizikalnih agensa prirodnog i antropogenog podrijetla [139892] Vilena Kašuba 022-0222148-2137 MZOS Flavonoidi i molekularni mehanizmi njihovih bioloških učinaka [164954] Gordana Rusak 119-1191192-1213 MZOS Dizajn, sinteza i ispitivanje interakcija malih molekula s DNA, RNA i proteinima [202026] Ivo Piantanida 098-0982914-2918 MZOS
Depositing User:	Ivo Piantanida
Date Deposited:	26 Nov 2014 08:32
URI:	http://fulir.irb.hr/id/eprint/1621
DOI:	10.1016/j.cbi.2010.07.008

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