Šešelja, Kate; Bazina, Iva; Vrecl, Milka; Farger, Jessica; Schicht, Martin; Paulsen, Friedrich; Baus Lončar, Mirela; Pirman, Tatjana (2023) Tff3 Deficiency Differentially Affects the Morphology of Male and Female Intestines in a Long-Term High-Fat-Diet-Fed Mouse Model. International Journal of Molecular Sciences, 24 (22). ISSN 1422-0067
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Abstract
Trefoil factor family protein 3 (Tff3) protects the gastrointestinal mucosa and has a complex mode of action in different tissues. Here, we aimed to determine the effect of Tff3 deficiency on intestinal tissues in a long-term high-fat-diet (HFD)-fed model. A novel congenic strain without additional metabolically relevant mutations (Tff3-/-/C57Bl6NCrl strain, male and female) was used. Wild type (Wt) and Tff3-deficient mice of both sexes were fed a HFD for 36 weeks. Long-term feeding of a HFD induces different effects on the intestinal structure of Tff3-deficient male and female mice. For the first time, we found sex-specific differences in duodenal morphology. HFD feeding reduced microvilli height in Tff3-deficient females compared to that in Wt females, suggesting a possible effect on microvillar actin filament dynamics. These changes could not be attributed to genes involved in ER and oxidative stress, apoptosis, or inflammation. Tff3-deficient males exhibited a reduced cecal crypt depth compared to that of Wt males, but this was not the case in females. Microbiome-related short-chain fatty acid content was not affected by Tff3 deficiency in HFD-fed male or female mice. Sex-related differences due to Tff3 deficiency imply the need to consider both sexes in future studies on the role of Tff in intestinal function.
| Item Type: | Article | ||||||||
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| Uncontrolled Keywords: | trefoil peptide 3; duodenum; cecum; high-fat diet; oxidative stress; ER stress; apoptosis; inflammation | ||||||||
| Subjects: | BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences BIOMEDICINE AND HEALTHCARE > Veterinary Medicine |
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| Divisions: | Division of Molecular Medicine | ||||||||
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| Depositing User: | Ivana Vuglec | ||||||||
| Date Deposited: | 01 Apr 2026 12:03 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/11558 | ||||||||
| DOI: | 10.3390/ijms242216342 |
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