Fourier Transform Infrared Spectroscopic Characterization of Aortic Wall Remodeling by Stable Gastric Pentadecapeptide BPC 157 After Unilateral Adrenalectomy in Rats

Smoday, Ivan Maria; Vukovic, Vlasta; Oroz, Katarina; Vranes, Hrvoje; Kalogjera, Luka; Gamulin, Ozren; Vlainić, Josipa; Milavic, Marija; Sikiric, Suncana; Nikolac Gabaj, Nora; Marijancevic, Domagoj; Koprivanac, Antun; Beketic Oreskovic, Lidija; Oreskovic, Ivana; Strbe, Sanja; Barisic, Ivan; Kordic, Mario; Tvrdeic, Ante; Seiwerth, Sven; Sikiric, Predrag; Boban Blagaic, Alenka; Skrtic, Anita (2026) Fourier Transform Infrared Spectroscopic Characterization of Aortic Wall Remodeling by Stable Gastric Pentadecapeptide BPC 157 After Unilateral Adrenalectomy in Rats. Pharmaceuticals, 19 (1). ISSN 1424-8247

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Abstract

Background: No Fourier transform infrared (FTIR) spectroscopy studies have directly evaluated adrenalectomy vessels, the technique’s established ability to probe collagen/elastin-associated spectral features and lipid peroxidation-related signatures, and protein structural damage. Stable gastric pentadecapeptide BPC 157 therapy was found to maintain the vascular function under severe stress, as FTIR spectroscopy recently demonstrated rapid peptide-induced molecular changes in healthy rat blood vessels, particularly in lipid content and protein secondary structure. Methods: To extend these findings and highlight the BPC 157 vascular background in the special circumstances of the course following unilateral adrenalectomy, abdominal aortas were collected at 15 min, 5 h, and 24 h after unilateral adrenalectomy for the FTIR spectroscopy assessment. Results: FTIR spectra were acquired, preprocessed, and analyzed using principal component analysis (PCA), support vector machine discriminant analysis (SVMDA), and band-specific statistics. BPC 157 (10 ng/kg intragatrically immediately after unilateral adrenalectomy) produced a clear, reproducible separation of aortic spectra from control samples at all time points. The main discriminatory spectral signatures were observed in three regions, including amide I and amide II (protein-related bands, consistent with collagen/elastin contributions) and lipid C–H stretching bands. These spectral signatures are consistent with early extracellular matrix reinforcement and membrane preservation in the vascular wall and align with the recovering effect on the lesions in counteraction of the severe vascular and multiorgan failure, attenuation/elimination of thrombosis and blood pressure disturbances in various occlusion/occlusion-like syndromes. Conclusions: Together, after unilateral adrenalectomy, the FTIR data provide molecular-level spectral signatures consistent with rapid remodeling of the aortic wall toward a more structurally stable and functionally favorable state.

Item Type:	Article
Uncontrolled Keywords:	FTIR spectroscopy; unilateral adrenalectomy; BPC 157; aortic wall remodeling
Subjects:	BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences BIOMEDICINE AND HEALTHCARE > Clinical Medical Sciences BIOMEDICINE AND HEALTHCARE > Clinical Medical Sciences > Clinical Pharmacology and Toxicology
Divisions:	Division of Molecular Medicine
Depositing User:	Ema Buhin Šaler
Date Deposited:	02 Feb 2026 11:19
URI:	http://fulir.irb.hr/id/eprint/11207
DOI:	10.3390/ph19010191

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