The Evaluation of γ-Zein Reduction Using Mass Spectrometry—The Influence of Proteolysis Type in Relation to Starch Degradability in Silages

Duvnjak, Marija; Butorac, Ana; Kljak, Kristina; Nišavić, Marija; Cindrić, Mario; Grbeša, Darko (2022) The Evaluation of γ-Zein Reduction Using Mass Spectrometry—The Influence of Proteolysis Type in Relation to Starch Degradability in Silages. Fermentation, 8 (551). ISSN 2311-5637

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Abstract

The starch availability and nutritional value of corn (Zea mays L.) are affected by zein proteins. The aim of the study was to see whether the proposed reduction of γ-zeins during the fermentation of silages is a result of either the enzymatic proteolytic activity or of the acidic environment, and how this reduction affects starch availability and degradability in high-moisture corn. A mass spectrometry (MS) technique was used to quantify the 16- and 27-kDa γ-zeins. Briefly, two-dimensional gel electrophoresis (2-DE) was used for γ-zein separation, followed by densitometry for protein quantification and matrix-assisted laser desorption ionization time- of-flight MS (MALDITOF/TOF) for protein identification. The results show that the reduction in γ-zeins induced by the ensiling led to a more pronounced starch availability and in vitro degradation, and this reduction was dependent on the type of proteolysis. More specifically, the results indicate that the reduction of γ-zeins in the ensiled corn was primarily driven by the enzymatic proteolysis. Furthermore, we demonstrated that 2-DE followed by densitometric quantification and the mass spectrometry analysis for protein identification can be used as a state-of-the-art method for γ- zein evaluation both in fresh and fermented/ensiled corn samples.

Item Type:	Article
Uncontrolled Keywords:	16–27 kDa γ-zeins ; in vitro starch degradability parameters ; high-moisture corn ; Lactiplantibacillus plantarum ; lactic acid ; MALDI mass spectrometry
Subjects:	NATURAL SCIENCES > Interdisciplinary Natural Sciences BIOTECHNICAL SCIENCES > Agronomy BIOTECHNICAL SCIENCES > Interdisciplinary Technical Sciences
Divisions:	Division of Molecular Medicine
Depositing User:	Mario Cindrić
Date Deposited:	03 Apr 2023 10:14
URI:	http://fulir.irb.hr/id/eprint/7811
DOI:	10.3390/fermentation8100551

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