hrvatski jezikClear Cookie - decide language by browser settings

Regulation of KRAS protein expression by miR-544a and KRAS-LCS6 polymorphism in wild-type KRAS sporadic colon adenocarcinoma

Marinović, Sonja; Škrtić, Anita; Catela Ivković, Tina; Poljak, Mirko; Kapitanović, Sanja (2021) Regulation of KRAS protein expression by miR-544a and KRAS-LCS6 polymorphism in wild-type KRAS sporadic colon adenocarcinoma. Human Cell, 34 (5). pp. 1455-1465. ISSN 1749-0774

| Request a personal copy from author
PDF - Submitted Version - article
Download (876kB) | Preview


Colorectal carcinoma (CRC) results from the accumulation of genetic mutations and alterations in signaling pathways. KRAS is mutated in 40% of CRC cases and is involved in increased tumor cells proliferation and survival. Although KRAS mutations are a dominant event in CRC tumorigenesis, increased wild-type KRAS expression has a similar efect on accelerated tumor growth. In this study, we investigated the KRAS status in correlation with clinicopathological features in sporadic CRC and more importantly the role of let- 7a-5p and miR- 544a-3p in the regulation of wild- type KRAS protein expression in the tumor center (T1) and invasive tumor front (T2). Analysis showed that 39.1% of tumor samples had KRAS mutations. In wild-type KRAS tumors, 62.0% were positive for KRAS protein expression and there was a higher percentage of KRAS-positive tumor cells and a higher intensity of immunohistochemical reaction in T2 than in T1 samples. This could not be attributed to differences in KRAS mRNA levels, suggesting regulation via miR-544a-3p expression which was signifcantly decreased in T2 samples. Furthermore, we demonstrated that tumor samples carrying the KRAS-LCS6 variant allele had signifcantly higher protein expression of the wild-type KRAS. Our results suggest the role of the KRAS-LCS6 polymorphism and miR-544a-3p expression in the regulation of wild-type KRAS protein expression in sporadic CRC.

Item Type: Article
Uncontrolled Keywords: Colon adenocarcinoma ; Immunohistochemistry ; KRAS ; let-7a ; miR-544a
Subjects: BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences > Human Genetics, Genomics and Proteomics
Divisions: Division of Molecular Medicine
Project titleProject leaderProject codeProject type
Mikrosatelitna nestabilnost (MSI i EMAST) u molekularnom profiliranju sporadičnih karcinoma debelog crijevaKapitanović, SanjaIP-2016-06-1430HRZZ
Depositing User: Sonja Marinović
Date Deposited: 08 Dec 2022 10:49
DOI: 10.1007/s13577-021-00576-2

Actions (login required)

View Item View Item


Downloads per month over past year

Increase Font
Decrease Font
Dyslexic Font