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Phenylboronic acid as a novel agent for controlling plant pathogenic bacteria

Martinko, Katarina; Ivanković, Siniša; Edyta, Đermić; Damir, Đermić (2022) Phenylboronic acid as a novel agent for controlling plant pathogenic bacteria. Pest management science, 78 (6). pp. 2417-2422. ISSN 1526-498X

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Abstract

Phenylboronic acid (PBA) is an environmentally non-toxic substance with antimicrobial activity. Due to increasing ecological limitations in phytopharmacy and considering the development of resistance of phytopathogenic bacteria to available antibacterial agents, here we explore a possible role of PBA as an antibacterial agent of choice. RESULTS We determined a minimal inhibitory concentration (MIC) of PBA in vitro on the Pseudomonas syringae pv. tomato (Pst) (0.5 mg/mL) and Erwinia amylovora (0.8 mg/mL), two of the most damaging plant pathogenic bacteria. In comparison, boric acid MIC was 2.5-6-fold higher than that of PBA, indicating enhanced antibacterial efficacy of the latter. Moreover, we determined the effect of PBA on cell growth and viability of both bacteria and have shown that PBA has bactericidal effect in concentrations > 1.0 mg/mL, whereas in lower concentration it is bacteriostatic. In addition, we have shown that PBA impairs Pst ability to cause symptoms on tomato plants in a dose- dependent manner, whereas solely applied PBA did not affect plant morphology at bactericidal concentrations. CONCLUSION We report, for the first time, that PBA is a suitable agent for controlling phytopathogenic bacteria. PBA has bacteriostatic activity in lower, and bactericidal activity in higher (> 1.0 mg/mL) concentrations. When applied on tomato plants, PBA managed to suppress symptoms caused by Pst, while having no adverse effect on plants at the bactericidal concentrations. As an additional benefit, PBA is environmentally friendly.

Item Type: Article
Uncontrolled Keywords: Erwinia amylovora ; MIC ; PBA ; Pseudomonas tomato ; bacterial speck
Subjects: NATURAL SCIENCES > Biology
NATURAL SCIENCES > Biology > Microbiology
BIOTECHNICAL SCIENCES > Agronomy > Phytomedicine
Divisions: Division of Molecular Biology
Division of Molecular Medicine
Depositing User: Damir Đermić
Date Deposited: 09 Nov 2022 11:48
URI: http://fulir.irb.hr/id/eprint/7608
DOI: 10.1002/ps.6872

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