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miR-7 AND miR-34a sequence cloning and expression in a1235 glioblastoma cell line

Kolić, Dora; Horvat, Luka; Šetinc, Maja; Antica, Mariastefania; Matulić, Maja (2020) miR-7 AND miR-34a sequence cloning and expression in a1235 glioblastoma cell line. Molecular and experimental biology in medicine, 3 (2). pp. 31-38. ISSN 2584-671X

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miRNAs are small non-coding RNAs which have an important role in signalling circuits regulating different cell processes. miR-7 and miR-34a are known as tumour suppressors, and both of them can interfere with cell proliferation, differentiation, apoptosis and migration. We constructed plasmids containing pri-miRNA sequences for these two miRNAs and introduced them into the A1235 glioblastoma cell line. Clones containing increased expression of processed miR-7 and miR-34a were obtained. The proliferation and sensitivity to alkylation agent of transfected cells were similar to those of control cells. Our results indicate that an increase in miR-7 and miR34 expression alone in A1235 glioblastoma cells is not sufficient to change their proliferation or sensitivity to the influence of alkylating agents.

Item Type: Article
Uncontrolled Keywords: miR - 7 ; miR - 34a ; expression ; glioblastoma cell line
Subjects: NATURAL SCIENCES > Biology
Divisions: Division of Molecular Biology
Project titleProject leaderProject codeProject type
Notch u diferencijaciji hematopoetskih stanica matica i u razvoju leukemijaAntica, MariastefaniaIP-2013-11-7140HRZZ
Depositing User: Mariastefania Antica
Date Deposited: 19 Jan 2021 08:44
DOI: 10.33602/mebm.3.2.4

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