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Interaction of α-Melanocortin and Its Pentapeptide Antisense LVKAT : Effects on Hepatoprotection in Male CBA Mice

Houra, Karlo; Turčić, Petra; Gabričević, Mario; Weitner, Tin; Konjevoda, Paško; Štambuk, Nikola (2011) Interaction of α-Melanocortin and Its Pentapeptide Antisense LVKAT : Effects on Hepatoprotection in Male CBA Mice. Molecules, 16 (9). pp. 7331-7343. ISSN 1420-3049

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The genetic code defines nucleotide patterns that code for individual amino acids and their complementary, i.e., antisense, pairs. Peptides specified by the complementary mRNAs often bind to each other with a higher specificity and efficacy. Applications of this genetic code property in biomedicine are related to the modulation of peptide and hormone biological function, selective immunomodulation, modeling of discontinuous and linear epitopes, modeling of mimotopes, paratopes and antibody mimetics, peptide vaccine development, peptidomimetic and drug design. We have investigated sense-antisense peptide interactions and related modulation of the peptide function by modulating the effects of a-MSH on hepatoprotection with its antisense peptide LVKAT. First, transcription of complementary mRNA sequence of a-MSH in 3’→5’ direction was used to design antisense peptide to the central motif that serves as a-MSH pharmacophore for melanocortin receptors. Second, tryptophan spectrofluorometric titration was applied to evaluate the binding of a-MSH and its central pharmacophore motif to the antisense peptide, and it was concluded that this procedure represents a simple and efficient method to evaluate sense-antisense peptide interaction in vitro. Third, we showed that antisense peptide LVKAT abolished potent hepatoprotective effects of a-MSH in vivo.

Item Type: Article
Uncontrolled Keywords: α-MSH ; antisense ; peptide ; fluorescence ; binding ; hepatoprotection
Subjects: NATURAL SCIENCES > Biology
Divisions: Division of Molecular Medicine
NMR Center
Project titleProject leaderProject codeProject type
Gastrin kao kofaktor unosa željeza u organizamMario Gabričević006-0061247-0978MZOS
Modeliranje bioaktivnih molekula i ispitivanje njihovih svojstava i učinkaNikola Štambuk098-0982929-2524MZOS
Depositing User: Nikola Štambuk
Date Deposited: 10 Mar 2020 15:12
DOI: 10.3390/molecules16097331

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