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The effect of copper and chlorpyrifos co-exposure on biomarkers in marine mussels Mytilus galloprovincialis

Perić, Lorena; Burić, Petra (2019) The effect of copper and chlorpyrifos co-exposure on biomarkers in marine mussels Mytilus galloprovincialis. Chemosphere, 225 . pp. 126-134. ISSN 0045-6535

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Metals and organophosphorous pesticides commonly co-occur in marine environment, but the effect of their mixtures on non-target organisms is still poorly understood. This study investigated the combined effect of the essential metal copper (Cu) and organophosphorous pesticide chlorpyrifos (Chp) in mussels Mytilus galloprovincialis after short-term exposure to their sublethal concentrations. Mussels were exposed for four days to 5 and 15 µg l-1 Cu and 0.05 and 5 µg l-1 Chp, and to their binary mixtures. The investigated biomarkers, namely acetylcholinesterase activity (AChE), glutathione S-transferase activity (GST), metallothioneins content (MTs) and lipid peroxide levels (LPO) displayed unspecific and inconsistent response patterns that varied depending on the concentration of chemicals and composition of mixtures. The exposure to Cu or Chp alone did not induce AChE activity changes, whereas only Cu provoked a significant GST activity increase. Exposure to lower and higher concentration of Chp resulted in MTs content and LPO level increase, respectively. Response of biomarkers to mixtures was generally inconsistent. Data integration by IBR index and PCA revealed different stress levels for given exposure conditions, but no explicit differentiation between single and joint exposures was found. The present results showed that low and environmentally relevant concentrations of Cu and Chp in mixtures may result in a detectable biological response, stressing the need for further investigation of joint effects of widespread marine contaminants in sentinel organisms.

Item Type: Article
Additional Information: This study was supported by the Ministry of Science and Education of the Republic of Croatia.
Uncontrolled Keywords: mixture, metals, pesticides, integrated biomarker response, environmentally relevant concentrations
Subjects: NATURAL SCIENCES > Interdisciplinary Natural Sciences > Marine Science
NATURAL SCIENCES > Interdisciplinary Natural Sciences > Environmental Science
Divisions: Center for Marine Research
Depositing User: Lorena Perić
Date Deposited: 23 Oct 2019 10:24
DOI: 10.1016/j.chemosphere.2019.03.003

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