In vitro characterization of zebrafish (Danio rerio) organic anion transporters Oat2a-e

Dragojević, Jelena; Mihaljević, Ivan; Popović, Marta; Žaja, Roko; Smital, Tvrtko (2017) In vitro characterization of zebrafish (Danio rerio) organic anion transporters Oat2a-e. Toxicology in Vitro, 46 . pp. 246-256. ISSN 0887-2333

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Abstract

OATS/Oats are transmembrane proteins that transport a variety of drugs, environmental toxins and endogenous metabolites into the cell. Zebrafish (Danio rerio) has seven OAT orthologs: Oat1, Oat2a–e and Oat3. In this study we specifically address Oat2 (Slc22a7) family. Conserved synteny analysis showed localization of zebrafish oat2 genes on two chromosomes, 11 and 17. All five zebrafish Oats were localized by live cell imaging in membranes of transiently transfected HEK293-T cells, and Oat2a, b, d, and e were confirmed using western blot analysis. Functional studies using the HEK293T cells overexpressing zebrafish Oats revealed two model fluorescent substrates of three Oats: Lucifer yellow for Oat2a and Oat2d (Km 122, and 49.7 μM), and 6- carboxyfluorescein for Oat2b and Oat2d (Km 199.7, and 266.9 μM). The initial screening of a series of diverse endo- and xenobiotics showed interaction with a number of compounds, including cGMP and diclofenac (IC50 27.74, and 19.14 μM) with Oat2a ; estrone-3-sulfate and diclofenac (IC50 30.96, and 12.6 μM) with Oat2b ; and fumarate and indomethacin (IC50 68.24, and 20.41 μM) with Oat2d. This study provides the first comprehensive data set on Oat2 in zebrafish and offers an important basis for more detailed molecular and (eco)toxicological characterizations of these transporters.

Item Type:	Article
Uncontrolled Keywords:	organic anion transporters (OATs) ; zebrafish ; Oat2-e ; functional characterization ; HepG2 cells ; transient transfection ; fluorescent substrates
Subjects:	NATURAL SCIENCES > Biology NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology NATURAL SCIENCES > Biology > Genetics, Evolution and Phylogenetics
Divisions:	Division for Marine and Enviromental Research
Projects:	Project title Project leader Project code Project type Identifikacija i funkcionalna karakterizacija (eko)toksikološki važnih polispecifičnih membranskih transportnih proteina u zebrici (Danio rerio)-TRANS-ZEBRATOX Tvrtko Smital IP-2013-11-4806 HRZZ
Depositing User:	Marta Popović
Date Deposited:	07 Nov 2018 16:20
URI:	http://fulir.irb.hr/id/eprint/4206
DOI:	10.1016/j.tiv.2017.09.026

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