Electrochemical characterization of biochemically active Cu(II) mixed ligand complex with histidine and cysteine

Mlakar, Marina; Pavković, Sonja; Tumir, Lidija- Marija; Vukosav, Petra; Gašparović, Blaženka (2017) Electrochemical characterization of biochemically active Cu(II) mixed ligand complex with histidine and cysteine. Electroanalysis, 29 (2). pp. 392-397. ISSN 1040-0397

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Abstract

Copper(II) complexes with cysteine and histidine, amino acids that coordinate copper(II) in human body, were investigated. Cu-His and Cu-Cys complexes were detected in pH range from 5.0 to 9.0 using voltammetric techniques. [CuHis2] complex reduces by two- electron reversible process at  -0.40 V, while [CuCys] complex by one-electron quasireversible process at -0.6 V, revealing strong adsorption at the electrode surface. When both amino acids are present in the solution, new peak appeared at -0.5 V, which corresponded to the [CuHisCys] complex reduction. Formation and characterization of mixed ligand complex was also supported by UV-Vis spectra recorded at fixed histidine and various cysteine concentrations. Formation of [CuHisCys] complex in the solution was detected and stability constant calculated to amount to logKCuHisCys=16.90.3. This study was the first attempt to characterize formation of Cu(II) mixed ligand complexation process with biochemically important amino acids in electron transport and oxygenation reactions in human body.

Item Type:	Article
Uncontrolled Keywords:	Copper ; histidine ; cysteine ; mixed ligand complexes ; voltammetry
Subjects:	NATURAL SCIENCES > Chemistry > Analytic Chemistry NATURAL SCIENCES > Interdisciplinary Natural Sciences > Marine Science
Divisions:	Division for Marine and Enviromental Research
Projects:	Project title Project leader Project code Project type Utjecaj okolišnog stresa na pojavnost i međudjelovanje biološki važnih organskih molekula i mikronutrijenata u morskom ekosustavu-AMBIOMERES Blaženka Gašparović IP-2013-11-8607 HRZZ
Depositing User:	Blaženka Gašparović
Date Deposited:	22 May 2018 08:44
URI:	http://fulir.irb.hr/id/eprint/4058
DOI:	10.1002/elan.201600253

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