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Copper-phospholipid interaction at cell membrane model hydrophobic surfaces

Mlakar, Marina; Cuculić, Vlado; Frka, Sanja; Gašparović, Blaženka (2018) Copper-phospholipid interaction at cell membrane model hydrophobic surfaces. Bioelectrochemistry, 120 (C). pp. 10-17. ISSN 1567-5394

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Detailed investigation of Cu (II) binding with natural lipid phosphatidylglycerol (PG) in aqueous solution was carried out by voltammetric measurements at the mercury drop electrode, complemented by monolayer studies in a Langmuir trough and electrophoretic measurements, all used as models for hydrophobic cell membranes. Penetration of copper ions into the PG layer was facilitated by the formation of hydrophilic Cu- Phenanthroline (Phen) complex in the subphase, followed by the mixed ligand Cu-Phen-PG complex formation at the hydrophobic interface. Electrophoretic measurements indicated a comparatively low abundance of the formed mixed ligand complex within the PG vesicles, resulting it the zeta potential change of + 0.83 mV, while monolayer studies confirmed their co-existence at the interface. The Cu- Phen-PG complex was identified in the pH range from 6 to 9. The stoichiometry of the complex ([PhenCuOHPG]), as well as its stability and kinetics of formation, were determined at the mercury drop electrode. Cu-Phen-PG reduces quasireversibly at about − 0.7 V vs. Ag/AgCl including reactant adsorption, followed by irreversible mixed complex dissociation, indicating a two-electron transfer - chemical reaction (EC mechanism). Consequently, the surface concentration (γ) of the adsorbed [PhenCuOHPG] complex at the hydrophobic electrode surface was calculated to be (3.35 ± 0.67) × 10− 11 mol cm− 2. Information on the mechanism of Cu (II) - lipid complex formation is a significant contribution to the understanding of complex processes at natural cell membranes.

Item Type: Article
Uncontrolled Keywords: Phospholipid membrane ; Copper (II) mixed ligand complex ; Voltammetry Langmuir monolayer ; Electrophoretic zeta potential
Subjects: NATURAL SCIENCES > Chemistry > Applied Chemistry
Divisions: Division for Marine and Enviromental Research
Project titleProject leaderProject codeProject type
Utjecaj okolišnog stresa na pojavnost i međudjelovanje biološki važnih organskih molekula i mikronutrijenata u morskom ekosustavu-AMBIOMERESBlaženka GašparovićIP-2013-11-8607HRZZ
Depositing User: Blaženka Gašparović
Date Deposited: 21 May 2018 13:31
DOI: 10.1016/j.bioelechem.2017.11.004

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