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Copper-phospholipid interaction at cell membrane model hydrophobic surfaces

Mlakar, Marina; Cuculić, Vlado; Frka, Sanja; Gašparović, Blaženka (2018) Copper-phospholipid interaction at cell membrane model hydrophobic surfaces. Bioelectrochemistry, 120 (C). pp. 10-17. ISSN 1567-5394

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Abstract

Detailed investigation of Cu (II) binding with natural lipid phosphatidylglycerol (PG) in aqueous solution was carried out by voltammetric measurements at the mercury drop electrode, complemented by monolayer studies in a Langmuir trough and electrophoretic measurements, all used as models for hydrophobic cell membranes. Penetration of copper ions into the PG layer was facilitated by the formation of hydrophilic Cu- Phenanthroline (Phen) complex in the subphase, followed by the mixed ligand Cu-Phen-PG complex formation at the hydrophobic interface. Electrophoretic measurements indicated a comparatively low abundance of the formed mixed ligand complex within the PG vesicles, resulting it the zeta potential change of + 0.83 mV, while monolayer studies confirmed their co-existence at the interface. The Cu- Phen-PG complex was identified in the pH range from 6 to 9. The stoichiometry of the complex ([PhenCuOHPG]), as well as its stability and kinetics of formation, were determined at the mercury drop electrode. Cu-Phen-PG reduces quasireversibly at about − 0.7 V vs. Ag/AgCl including reactant adsorption, followed by irreversible mixed complex dissociation, indicating a two-electron transfer - chemical reaction (EC mechanism). Consequently, the surface concentration (γ) of the adsorbed [PhenCuOHPG] complex at the hydrophobic electrode surface was calculated to be (3.35 ± 0.67) × 10− 11 mol cm− 2. Information on the mechanism of Cu (II) - lipid complex formation is a significant contribution to the understanding of complex processes at natural cell membranes.

Item Type: Article
Uncontrolled Keywords: Phospholipid membrane ; Copper (II) mixed ligand complex ; Voltammetry Langmuir monolayer ; Electrophoretic zeta potential
Subjects: NATURAL SCIENCES > Chemistry > Applied Chemistry
Divisions: Division for Marine and Enviromental Research
Projects:
Project titleProject leaderProject codeProject type
Utjecaj okolišnog stresa na pojavnost i međudjelovanje biološki važnih organskih molekula i mikronutrijenata u morskom ekosustavu-AMBIOMERESBlaženka GašparovićIP-2013-11-8607HRZZ
Depositing User: Blaženka Gašparović
Date Deposited: 21 May 2018 13:31
Last Modified: 21 May 2018 13:31
URI: http://fulir.irb.hr/id/eprint/4041
DOI: 10.1016/j.bioelechem.2017.11.004

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