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Detection of correlated conformational fluctuations in intrinsically disordered proteins through paramagnetic relaxation interference

Kurzbach, Dennis; Vanas, Agathe; Flamm, Andrea G.; Tarnoczi, Nicole; Kontaxis, Georg; Maltar-Strmečki, Nadica; Widder, Katharina; Hinderberger, Dariush; Konrat, Robert (2016) Detection of correlated conformational fluctuations in intrinsically disordered proteins through paramagnetic relaxation interference. Physical Chemistry Chemical Physics, 18 (8). pp. 5753-5758. ISSN 1463-9076

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Abstract

Functionally relevant conformational states of intrinsically disordered proteins (IDPs) are typically concealed in a vast space of fast interconverting structures. Here we present a novel methodology, NMR-based paramagnetic relaxation interference (PRI), that allows for direct observation of concerted motions and cooperatively folded sub-states in IDPs. The proposed NMR technique is based on the exploitation of cross correlated electron- nuclear dipolar relaxation interferences in doubly spin- labeled proteins and probes the transient spatial encounter of electron-nucleus spin pairs.

Item Type: Article
Additional Information: DK thanks the Alexander von Humboldt Foundation for a Feodor-Lynen scholarship. This work was supported in part by the FWF (P26317-B21 and W-1221-B03).
Uncontrolled Keywords: disordered proteins; relaxation; NMR-based paramagnetic relaxation interference
Subjects: NATURAL SCIENCES > Physics
NATURAL SCIENCES > Chemistry
Divisions: Division of Physical Chemistry
Projects:
Project titleProject leaderProject codeProject type
Alexander von Humboldt FoundationUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
FWFUNSPECIFIEDP26317-B21UNSPECIFIED
FWFUNSPECIFIEDW-1221-B03UNSPECIFIED
Depositing User: Nadica Maltar Strmečki
Date Deposited: 14 Dec 2016 14:22
Last Modified: 14 Dec 2016 15:47
URI: http://fulir.irb.hr/id/eprint/3214
DOI: 10.1039/C5CP04858C

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