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Protein adsorption on various plasma-treated polyethylene terephthalate substrates

Recek, Nina; Jaganjac, Morana; Kolar, Metod; Milković, Lidija; Mozetič, Miran; Stana-Kleinschek, Karin; Vesel, Alenka (2013) Protein adsorption on various plasma-treated polyethylene terephthalate substrates. Molecules, 18 (10). pp. 12441-12463. ISSN 1420-3049

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Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF4 plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy (SEM). The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates.

Item Type: Article
Uncontrolled Keywords: oxygen and fluorine plasma treatment; polymer surface modification; protein adsorption; cell adhesion; quartz crystal microbalance (QCM)
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Division of Molecular Medicine
Project titleProject leaderProject codeProject type
Lipidi, slobodni radikali i njihovi glasnici u integrativnoj onkologiji-Neven ZARKOVIC098-0982464-2519MZOS
Depositing User: Lidija Milković
Date Deposited: 07 Sep 2016 14:29
DOI: 10.3390/molecules181012441

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