Monitoring lipid phase transition temperatures using fluorescent probes and temperature-dependent fluorescence spectroscopy

Brkljača, Zlatko; Čehić, Mirsada; Portada, Tomislav; Butumović, Marija; Bakarić, Danijela; Crnolatac, Ivo (2022) Monitoring lipid phase transition temperatures using fluorescent probes and temperature-dependent fluorescence spectroscopy. Dyes and Pigments, 206 . ISSN 0143-7208

| Request a personal copy from author

Abstract

The response of fluorescent dyes incorporated in multilamellar liposomes was evaluated for their applicability in monitoring thermal-induced changes in liposomes. Five fluorescent probes, diverse in polarity, lipophilicity, and consequently their position within the lipid multibilayers constituted from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), were used to record temperature-dependent fluorescence spectra of the liposomal suspensions. The responses of the dyes were analysed in a multivariate fashion to determine pre- and main phase transition temperatures of DPPC. Laurdan® and 1-chloro-9,10-bis(phenylethynyl)anthracene register both phase transitions, while 9,10-diphenylanthracene and 9,10-bis(phenylethynyl)anthracene report only pretransition. According to the differential scanning calorimetry (DSC) measurements, all suspensions display both phase transitions that resemble DSC curve of pure DPPC. The positions of the dyes within the bilayers were determined by molecular dynamics simulations.

Item Type:	Article
Uncontrolled Keywords:	1, 2-Dipalmitoyl-sn-glycero-3-phosphocholine ; Fluorescent dyes ; Differential scanning calorimetry ; Fluorescence spectroscopy ; Multivariate curve analysis ; Molecular dynamics simulations
Subjects:	NATURAL SCIENCES NATURAL SCIENCES > Chemistry NATURAL SCIENCES > Chemistry > Physical Chemistry NATURAL SCIENCES > Chemistry > Analytic Chemistry
Divisions:	Division of Organic Chemistry and Biochemistry Division of Physical Chemistry
Projects:	Project title Project leader Project code Project type Model demijelinizacije na molekulskoj skali pri fiziološkim i patološkim uvjetima-DEMYMOLSCALE Danijela Bakarić UIP-2020-02-7669 HRZZ Identifikacija interaktoma paralognih proteina SSB u višestaničnom prokariotu, Streptomyces coelicolor-id_ROBUST Dušica Vujaklija IP-2018-01-1754 HRZZ
Depositing User:	Danijela Bakarić
Date Deposited:	07 Jan 2026 14:39
URI:	http://fulir.irb.hr/id/eprint/10700
DOI:	10.1016/j.dyepig.2022.110621

Actions (login required)

View Item