The impact and complete genome characterisation of viruses involved in outbreaks of gastroenteritis in a farrow-to-finish holding

Brnić, Dragan; Vlahović, Dunja; Gudan Kurilj, Andrea; Maltar-Strmečki, Nadica; Lojkić, Ivana; Kunić, Valentina; Jemeršić, Lorena; Bačani, Ivica; Kompes, Gordan; Beck, Relja; Mikuletič, Tina; Steyer, Andrej (2023) The impact and complete genome characterisation of viruses involved in outbreaks of gastroenteritis in a farrow-to-finish holding. Scientific Reports, 13 (1). ISSN 2045-2322

PDF - Objavljena/završna verzija - članak Download (5MB)

Sažetak

Viral enteric pathogens continuously burden intensive pig farming, causing gastrointestinal diseases of epidemic and endemic nature. The present study investigated two diarrhoea outbreaks on a large farrow-to-finish holding and subsequent circulation of outbreak-related enteric viruses. These viruses were characterised by whole genome sequencing, and statistical evaluation of the impact on specific production metrics was performed. The results provided evidence that the Porcine epidemic diarrhoea virus–swine enteric coronavirus (PEDV–SeCoV) S gene recombinant strain was responsible for the first outbreak, whilst Rotavirus A (RVA) in a mixed infection with Rotavirus B (RVB) and porcine kobuvirus (PKV) probably caused the second diarrhoea outbreak. Whole genome characterisation revealed a porcine origin of all viruses involved and significant heterogeneity of RVB strain, proposing four novel genotypes and changes in RVB VP1 genotype classification. The statistical evaluation confirmed only a minor disturbance in the number of weaned pigs per sow, with statistical forecasting showing positive trends. A follow-up study corroborated the endemicity of RVA and PKV, in contrast to PEDV–SeCoV. Punctual, comprehensive and timely investigation of diarrhoea outbreaks is a prerequisite for applying adequate pig health and biosecurity management. Calculating such outbreaks' impact on production metrics can potentially shape future decisions on management improvements.

Vrsta građe:	Rad objavljen u časopisu
Ključne riječi:	PORCINE EPIDEMIC DIARRHEASWINE ENTERIC CORONAVIRUSHUNGARYSYSTEMFECESPIGSPCR
Područja:	PRIRODNE ZNANOSTI > Fizika PRIRODNE ZNANOSTI > Kemija BIOMEDICINA I ZDRAVSTVO > Javno zdravstvo i zdravstvena zaštita BIOMEDICINA I ZDRAVSTVO > Veterinarska medicina > Veterinarsko javno zdravstvo i sigurnost hrane
Zavodi:	Zavod za fizičku kemiju
Projekti:	Naziv projekta Voditelj projekta Šifra projekta Vrsta projekta Rotavirusi u ekosustavu Republike Hrvatske: molekularna epidemiologija i zoonotski potencijal-REco Dragan Brnić UIP-2017-05-8580 HRZZ NEODREĐENO NEODREĐENO D NEODREĐENO
Pohranio:	Nadica Maltar Strmečki
Datum pohrane:	06 Feb 2024 12:38
URI:	http://fulir.irb.hr/id/eprint/8511
DOI:	10.1038/s41598-023-45994-4

Actions (login required)

Pokaži zapis