Encapsulation of Vecuronium and Rocuronium by Sugammadex Investigated by Surface-Enhanced Raman Spectroscopy

Kenđel, Adriana; Piantanida, Ivo; Miljanić, Snežana (2025) Encapsulation of Vecuronium and Rocuronium by Sugammadex Investigated by Surface-Enhanced Raman Spectroscopy. Molecules, 30 (2). ISSN 1420-3049

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Abstract

Aiming toward a novel, non-invasive technique, with real-time potential application mon-itoring of complexation of steroidal neuromuscular blocker drugs Vecuronium (Vec ) and Rocuronium (Roc) with sugammadex (SDX, medication for the reversal of neuro-muscular blockade induced by Vec or Roc in general anesthesia), we developed proof-of-principle methodology based on surface-enhanced Raman spectroscopy (SERS). Silver nanoparticles prepared by reduction of silver ions with hydroxylamine hydrochlo-ride were used as SERS active substrates, additionally aggregated with calcium nitrate as needed. The Vec and Roc drugs SERS spectra were obtained within the biorelevant 5×10−7–1×10−4 M range, as well as the SERS of SDX, latter observed only in the presence of the aggregating agent. SDX/drug complexes at 1/1 molar ratio revealed significant spectral changes in vibrational bands of the SDX glucose rings and the drug steroid rings, imply-ing that the insertion of Vec and Roc molecules into the SDX cavity was not only driven by attractive electrostatic interactions between the positively charged cyclic unit of the drug and the negative carboxylate groups of cyclodextrin, but also supported by hydro-phobic interactions between the host cyclodextrin and the guest drug molecule. Observed changes in SERS signals are applicable in biorelevant conditions and support further studies of SDX/drug complexes in vivo.

Item Type:	Article
Uncontrolled Keywords:	vecuronium; rocuronium; γ-cyclodextrin; sugammadex; SERS
Subjects:	NATURAL SCIENCES > Chemistry > Analytic Chemistry
Divisions:	Division of Organic Chemistry and Biochemistry
Depositing User:	Ana Zečević
Date Deposited:	29 Apr 2025 10:22
URI:	http://fulir.irb.hr/id/eprint/9779
DOI:	10.3390/molecules30020231

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