Maleš, Petra; Pem, Barbara; Bakarić, Danijela (2025) Modulation of thermotropic properties of cholesterol-free model myelin membranes by myelin basic protein. In: Saveljić, Igor; Filipović, Nenad, (eds.) 3rd International Conference on Chemo and BioInformatics - Book of Proceedings. pp. 48-52 .
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Abstract
As a continuation of our research on model myelin membranes (MMM), in which we revealed that MMM that mimic normal (healthy) myelin are more fluid at 30 °C than those that mimic modified (diseased) myelin, we aimed to unveil the contribution of cholesterol in MMM thermotropic properties. Accordingly, we prepared MMM as lipid mixtures in the presence and absence of myelin basic protein (MBP), but excluding cholesterol (named MMM’). Using differential scanning calorimetry (DSC) and temperature-dependent UV-Vis spectroscopy, we determined the main phase transition temperature (Tm) of MMM’, i.e., the temperature of the gel-to-fluid phase transition of lipids in MMM’ systems. Besides the discrepancies obtained by these two techniques, we identified a difference of about 5 °C between normal and modified MMM’, independently of MBP. As evidenced by the maintenance and weakening of van der Waals forces at 30 °C and 60 °C, respectively, the fluidity of normal and modified MMM’ is comparable at a corresponding temperature. The modified MMM’ reflected different polar headgroup hydration than normal MMM’ at 30 °C, which is very likely associated with the stabilization of the gel phase in modified MMM’.
| Item Type: | Conference or workshop item published in conference proceedings (UNSPECIFIED) | ||||||||
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| Uncontrolled Keywords: | model myelin membranes; cholesterol; myelin basic protein; thermotropic properties | ||||||||
| Subjects: | NATURAL SCIENCES > Chemistry > Physical Chemistry | ||||||||
| Divisions: | Division of Organic Chemistry and Biochemistry | ||||||||
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| Depositing User: | Danijela Bakarić | ||||||||
| Date Deposited: | 09 Jan 2026 14:58 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/10809 | ||||||||
| DOI: | 10.46793/ICCBIKG25.048M |
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