(Un)coupling the factors contributing to the interfacial activation of Streptomyces rimosus lipase: computational and spectrophotometric study

Čehić, Mirsada; Brkljača, Zlatko; Filić, Želimira; Crnolatac, Ivo; Vujaklija, Dušica; Bakarić, Danijela (2024) (Un)coupling the factors contributing to the interfacial activation of Streptomyces rimosus lipase: computational and spectrophotometric study. Journal of Dispersion Science and Technology, 45 (2). pp. 296-306. ISSN 0193-2691

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Official URL: https://www.tandfonline.com/doi/full/10.1080/01932...

Abstract

Streptomyces rimosus lipases (SrLip) present an enzyme class that catalyses the hydrolysis of triacylglycerols producing fatty acids and glycerols. The abrupt increase in catalytic activity of SrLip when found at water-oil interface is assigned as interfacial activation (IA). Since the latter is a complex function of interface charge, chemical composition, as well as substrate orientation and concentration, the role of individual factors and their combinations that trigger IA remains rather elusive. The aim of this work is to unravel the contribution of particular factor in IA by examining SrLip activity towards p-nitrophenylpalmitate (p-NPP) as a substrate incorporated in three chemically different self- assembled aggregates: gum Arabic, sodium deoxycholate and liposomes made of 1, 2-dioleyl- sn- glycero-3-phosphocholine (DOPC) lipids, and at two different pH values (pH = 6.8 and pH = 10.0). Spectrophotometric (UV/Vis) study revealed that SrLip catalyses p-NPP hydrolysis when the substrate is embedded in sodium deoxycholate at both pH values, suggesting that the coupling of favorable interface charge and substrate orientation drives the interfacial activation. Moreover, especially in alkaline conditions (pH = 10.0), hydrolysis of the ester bond of the substrate incorporated in DOPC bilayers occurs when appropriate orientation, concentration and arrangement is accomplished. All experiments were conducted in parallel on an inactive mutant SrLipS10/A as a reference. The molecular-level details of particular interface and accompanied substrates examined by all-atom molecular dynamics simulations revealed that availability of p-NPP at these three interfaces decreases in the following order: sodium-deoxycholate > DOPC > model gum Arabic.

Item Type:

Article

Uncontrolled Keywords:

p-nitrophenylpalmitate (p-NPP); Streptomyces rimosus lipase (SrLip); Interfacial activation (IA); Gum Arabic; Sodium deoxycholate; 1, 2-Dioleyl-sn-glycero-3-phosphocholine (DOPC) liposomes

Subjects:

NATURAL SCIENCES
NATURAL SCIENCES > Chemistry
NATURAL SCIENCES > Chemistry > Physical Chemistry

Divisions:

Division of Organic Chemistry and Biochemistry
Division of Physical Chemistry

Projects:

Project title	Project leader	Project code	Project type
Model demijelinizacije na molekulskoj skali pri fiziološkim i patološkim uvjetima-DEMYMOLSCALE	Danijela Bakarić	UIP-2020-02-7669	HRZZ
Identifikacija interaktoma paralognih proteina SSB u višestaničnom prokariotu, Streptomyces coelicolor-id_ROBUST	Dušica Vujaklija	IP-2018-01-1754	HRZZ
Višekromoforne probe za prepoznavanje pojedinih struktura DNA, RNA i proteina-BioMultiChromoProbes	Ivo Piantanida	IP-2018-01-5475	HRZZ