Deep-freezing and decontamination strategy for a large autologous bone graft with presentation of the osteogenic potential of resident osteoblasts

Zekušić, Marija; Skoko, Marija; Vukušić, Kruno; Ledinski, Maja; Bujić Mihica, Marina; Blažević, Dejan; Sošić, Zlata A.; Đura, Mario; Martinčić, Jelena; Vrgoč Zimić, Ivana; Batarilo, Ivanka; Polančec, Denis; Zenić, Lucija; Ramić, Snježana; Sesar, Patricija; Crkvenac Gornik, Kristina; Babić, Slaven; Pavešić, Jure; Sabalić, Srećko; Tolić, Iva M.; Urlić, Inga; Misir Šitum, Stanka; Vučemilo, Tiha; Vidović, Dinko (2025) Deep-freezing and decontamination strategy for a large autologous bone graft with presentation of the osteogenic potential of resident osteoblasts. Stem Cell Research & Therapy, 17 . ISSN 1757-6512

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Abstract

Musculoskeletal injuries resulting from high-impact trauma often lead to extensive bone defects that require effective reconstruction to prevent limb amputation. However, contamination of large autologous bone grafts in open fractures remains a major clinical challenge. This study aimed to establish a reliable protocol for decontamination of massive autologous bone graft and to assess the viability and regenerative potential of osteoblasts from frozen bone, including the effects of deep freezing and the cryoprotectant dimethyl sulfoxide (DMSO).

Item Type:	Article
Uncontrolled Keywords:	decontamination; autologous bone; femoral heads; analysis of antibiotic residues
Subjects:	BIOMEDICINE AND HEALTHCARE
Divisions:	Division of Molecular Biology
Projects:	Project title Project leader Project code Project type Molecular origins of aneuploidies in healthy and diseased human tissues-ANEUPLOIDY Iva Tolić; Nenad Pavin 855158 EK Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini-IPSted Iva Tolić KK.01.1.1.04.0057 EK Provedba vrhunskih istraživanja u sklopu Znanstvenog centra izvrsnosti za kvantne i kompleksne sustave te reprezentacije Liejevih algebri-QuantiXLie Hrvoje Buljan; Pavle Pandžić; Vit Tuček; Tomislav Marketin KK.01.1.1.01.0004 EK
Depositing User:	Iva Marija Tolić
Date Deposited:	07 Jan 2026 14:44
URI:	http://fulir.irb.hr/id/eprint/10741
DOI:	10.1186/s13287-025-04839-9

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