Construction of the Arabidopsis isogenic lines containing dually localized protein TROL only in the inner chloroplast envelope membrane

Vojta, Lea; Fulgosi, Hrvoje; Tomašić Paić, Ana; Dumančić, Ena (2025) Construction of the Arabidopsis isogenic lines containing dually localized protein TROL only in the inner chloroplast envelope membrane. Acta Botanica Croatica, 84 (2). pp. 256-265. ISSN 1847-8476

PDF - Published Version - article Available under License Creative Commons Attribution. Download (2MB)

Abstract

The thylakoid rhodanese-like protein (TROL) is located at the end of the photosynthetic electron transport chain, at the vicinity of photosystem I, where it dynamically interacts with the ferredoxin:NADP+ oxidoreductase (FNR) and is postulated to facilitate the transfer of electrons from reduced ferredoxin (Fd) to NADP+. TROL is one of the few so far known dually localized chloroplast proteins. Besides being localized in the thylakoid membranes as the 66 kDa mature form, it has also been found in the inner envelope membrane of chloroplasts as the 70 kDa precursor. In thylakoids, the interaction between TROL and FNR acts like a switch, prioritizing the photosynthetic electron destination sinks according to the organellar needs. The role of TROL in the chloroplast inner envelope membrane is, however, presently unknown. By engineering the presequence protease processing site, a single amino acid exchange of Ala67 to Ile67 has been introduced to TROL, leading to inhibited cleavage of the presequence and resulting in protein incorporation at the inner envelope membrane. In this work, we engineered the Arabidopsis mutant plants that contain TROL almost exclusively in the inner envelope membrane (TROL-IE). To facilitate studying the role of this protein in this chloroplast compartment, we also produced the antiserum specific for the IE form of the TROL.

Item Type:	Article
Uncontrolled Keywords:	dual localization; inner envelope membrane; mutagenesis; photosynthesis; presequence processing; thylakoids
Subjects:	NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology
Divisions:	Division of Molecular Biology
Projects:	Project title Project leader Project code Project type Redox-sensing and electron transfer at the inner envelope of chloroplasts – the role of the TROL-FNR interaction-ICGEB TROL-FNR - VOJTA Lea Vojta CRP/HRV20-03 UNSPECIFIED
Depositing User:	Lea Vojta
Date Deposited:	11 Dec 2025 15:10
URI:	http://fulir.irb.hr/id/eprint/10398
DOI:	10.37427/botcro-2025-006

Actions (login required)

View Item