Characterisation of Plasmid-Associated Antimicrobial Resistance Genes in Coastal Marine Enterobacterales from the Central Adriatic Sea: De Novo Assembly and Bioinformatic Profiling

Šamanić, Ivica; Dželalija, Mia; Bellulovich, Ema; Kalinić, Hrvoje; Jozić, Slaven; Ordulj, Marin; Udiković-Kolić, Nikolina; Maravić, Ana (2025) Characterisation of Plasmid-Associated Antimicrobial Resistance Genes in Coastal Marine Enterobacterales from the Central Adriatic Sea: De Novo Assembly and Bioinformatic Profiling. International Journal of Molecular Sciences, 26 (22). ISSN 1422-0067

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Abstract

This study examines the genomic composition and resistance potential of eight putative plasmid-derived contig assemblies reconstructed from marine Enterobacterales isolated in the central Adriatic Sea. Using a combination of Illumina-based whole genome sequencing, de novo assembly, and a multi-tool bioinformatics pipeline, we annotated antimicrobial resistance genes (ARGs), insertion sequences (ISs), and plasmid replicon types. Clinically significant resistance markers such as blaKPC, blaTEM, aacA4, tetA, and folP were identified, frequently co-localised with mobile genetic elements including IS110, IS4, and IS1182. The plasmid-associated contigs were assigned to MOBP and MOBQ types and contained replicon markers (IncP6, IncA/C2) characteristic of broad-host-range plasmids. Our findings provide valuable insight into the plasmidome of environmental Enterobacterales, emphasising the role of coastal pollution in shaping the distribution and potential mobility of antimicrobial resistance genes. This supports the One Health framework by linking environmental reservoirs to clinically relevant resistance mechanisms.

Item Type:	Article
Uncontrolled Keywords:	plasmid-associated resistance; coastal Enterobacterales; Adriatic Sea; mobile genetic elements; replicon typing; One Health
Subjects:	BIOTECHNICAL SCIENCES > Biotechnology > Molecular Biotechnology INTERDISCIPLINARY FIELDS OF ART
Divisions:	Division for Marine and Enviromental Research
Projects:	Project title Project leader Project code Project type Sezonska i prostorna raspodjela gena rezistencije na antibiotike u morskim mikrobnim zajednicama duž trofičkog gradijenta u srednjem Jadranu-ARGAS Ana Maravić UIP-2019-04-9778 HRZZ
Depositing User:	Nikolina Udiković Kolić
Date Deposited:	10 Dec 2025 14:13
URI:	http://fulir.irb.hr/id/eprint/10300
DOI:	10.3390/ijms262210910

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