Dumančić, Ena; Vojta, Lea; Fulgosi, Hrvoje (2025) Confocal Laser Scanning Microscopy of Light-Independent ROS in Arabidopsis thaliana (L.) Heynh. TROL-FNR Mutants. International Journal of Molecular Sciences, 26 (14). ISSN 1422-0067
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Abstract
Thylakoid rhodanese-like protein (TROL) serves as a thylakoid membrane hinge linking photosynthetic electron transport chain (PETC) complexes to nicotinamide adenine dinucleotide phosphate (NADPH) synthesis. TROL is the docking site for the flavoenzyme ferredoxin-NADP+ oxidoreductase (FNR). Our prior work indicates that the TROL-FNR complex maintains redox equilibrium in chloroplasts and systemically in plant cells. Improvement in the knowledge of redox regulation mechanisms is critical for engineering stress-tolerant plants in times of elevated global drought intensity. To further test this hypothesis and confirm our previous results, we monitored light-independent ROS propagation in the leaves of Arabidopsis wild type (WT), TROL knock-out (KO), and TROL ΔRHO (RHO-domain deletion mutant) mutant plants in situ by using confocal laser scanning microscopy with specific fluorescent probes for the three different ROS: O2·−, H2O2, and 1O2. Plants were grown under the conditions of normal substrate moisture and under drought stress conditions. Under the drought stress conditions, the TROL KO line showed ≈32% less O2·− while the TROL ΔRHO line showed ≈49% less H2O2 in comparison with the WT. This research confirms the role of dynamical TROL-FNR complex formation in redox equilibrium maintenance by redirecting electrons in alternative sinks under stress and also points it out as promising target for stress-tolerant plant engineering.
| Item Type: | Article | ||||||||
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| Uncontrolled Keywords: | abiotic stress; Arabidopsis; ferredoxin:NADP+; oxidoreductase; linear electron transport; redox homeostasis | ||||||||
| Subjects: | NATURAL SCIENCES > Biology NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology BIOTECHNICAL SCIENCES > Biotechnology |
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| Divisions: | Division of Molecular Biology | ||||||||
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| Depositing User: | Lorena Palameta | ||||||||
| Date Deposited: | 04 Sep 2025 08:36 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/9967 | ||||||||
| DOI: | 10.3390/ijms26147000 |
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