Enhancing ex situ cultivation of Mediterranean Fucales: species-specific responses of Gongolaria barbata and Ericaria crinita seedlings to algal extracts

Lokovšek, Ana; Orlando-Bonaca, Martina; Gljušćić, Edi; Bilajac, Andrea; Iveša, Ljiljana; Di Cave, Alberta; Savio, Saverio; Ortenzi, Federico; Trkov, Domen; Congestri, Roberta; Falace, Annalisa (2025) Enhancing ex situ cultivation of Mediterranean Fucales: species-specific responses of Gongolaria barbata and Ericaria crinita seedlings to algal extracts. Marine Environmental Research, 211 . ISSN 0141-1136

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Abstract

Mediterranean brown algal forests, dominated by Cystoseira sensu lato species, are undergoing widespread decline due to the cumulative effects of anthropogenic pressure and climate-related stressors. Restoration efforts increasingly rely on ex situ cultivation and outplanting of seedlings, yet early developmental stages often suffer from low survival and growth rates. This study investigated the potential of algal extracts to enhance the seedling development and survival in two canopy-forming species, Gongolaria barbata and Ericaria crinita. We tested extracts from a cyanobacterium (Trichormus variabilis), two microalgae (Desmodesmus sp. and Cylindrotheca closterium), and a commercial macroalgal formulation (AlgatronCifo®) at varying concentrations under controlled mesocosm conditions. Seedling performance was significantly influenced by extract type, and target species identity. Notably, a low-concentration Desmodesmus sp. extract (0.07 mg mL−1) improved survival and growth, whereas T. variabilis exerted an inhibitory effect on G. barbata. AlgatronCifo® did not outperform Desmodesmus sp. extract in promoting seedling development. These findings suggest that specific extracts from green microalgae could improve protocols for the early stages of restoration, offering a scalable tool for reha- bilitating degraded marine forests. However, the results underscore the importance of species-specific optimi- zation and the need for in situ validation of biostimulant-based restoration approaches.

Item Type:	Article
Uncontrolled Keywords:	Cystoseira s.l.; Seaweed biostimulants; Microalgal extracts; Seedling development; Restoration; Mediterranean sea
Subjects:	NATURAL SCIENCES
Divisions:	Center for Marine Research
Projects:	Project title Project leader Project code Project type Odgovori smeđih algi roda Cystoseira, koje formiraju staništa, na lokalne i globalne stresore Ljiljana Iveša IP-2019-04-6984 HrZZ Ispitivanje in-situ i ex-situ tehnika obnove naselja smeđih algi reda Fucales Ljiljana Iveša IP-2024-05-2362 HrZZ
Depositing User:	Lorena Palameta
Date Deposited:	03 Sep 2025 12:31
URI:	http://fulir.irb.hr/id/eprint/9960
DOI:	10.1016/j.marenvres.2025.107411

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