Charged Thienobenzo-1,2,3-Triazoles as Especially Potent Non-Selective Cholinesterase Inhibitors: Design, Anti-Inflammatory Activity, and Computational Study

Jelčić, Antonija; Raspudić, Anamarija; Barić, Danijela; Ratković, Ana; Šagud, Ivana; Pongrac, Paula; Štefok, Dora; Bosnar, Martina; Roca, Sunčica; Lasić, Zlata; Odak, Ilijana; Škorić, Irena (2025) Charged Thienobenzo-1,2,3-Triazoles as Especially Potent Non-Selective Cholinesterase Inhibitors: Design, Anti-Inflammatory Activity, and Computational Study. Pharmaceuticals, 18 (7). ISSN 1424-8247

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Abstract

Background/Objectives: This research reports the synthesis and evaluation of novel charged thienobenzo-triazoles as non-selective cholinesterase inhibitors (AChEs and BChEs), their anti-inflammatory properties, and a computational study. Methods: Fifteen derivatives were created through photochemical cyclization and quaternization of the triazole core. The compounds were tested for AChE and BChE inhibition. They showed greater potency and selectivity toward BChE. Results: The most potent compound, derivative 14, inhibited BChE with an IC50 of 98 nM, while derivative 9 also displayed significant anti-inflammatory activity by inhibiting LPS-induced TNF-α production (IC50 = 0.66 µM). Molecular docking revealed that triazolinium salts form key π-π and electrostatic interactions within enzyme active sites. In silico predictions indicated favorable ADME-Tox properties for compounds 9 and 11, including low mutagenicity and moderate CNS permeability. Conclusions: These findings highlight the potential of new charged triazolinium salts as peripherally selective cholinesterase inhibitors with additional anti-inflammatory potential.

Item Type:	Article
Uncontrolled Keywords:	thienobenzo-1,2,3-triazoles; cholinesterase; BChE inhibitors; anti-inflammatory activity; molecular docking; ADME-Tox prediction
Subjects:	NATURAL SCIENCES
Divisions:	Division of Physical Chemistry NMR Center
Depositing User:	Lorena Palameta
Date Deposited:	30 Jul 2025 07:42
URI:	http://fulir.irb.hr/id/eprint/9929
DOI:	10.3390/ph18071032

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