Muck-Šeler, Dorotea; Peričić, Danka
Binding of dihydroergosine to 5-HT1A receptors of human and rat brain.
Croatica Chemica Acta, 75
Interaction of the ergot alkaloid dihydroergosine with the binding of (H-3)8-hydroxy-2-(di-n-propylamino)tetralin ((3)H8-OH-DPAT), a selective agonist for 5-HT1A binding sites, to hippocampal membranes isolated from human and rat brain was studied. Competition binding experiments showed that dihydroergosine is a potent displacer of (H-3)8-OH-DPAT binding at brain 5-HT1A receptors of both species. Scatchard analysis of (H-3)8-OH-DPAT binding to rat hippocampal membranes in the presence of dihydroergosine revealed that this ergot compound markedly decreases the number and the affinity of hippocampal (H-3)8-OH-DPAT labelled binding sites. Preincubation of rat hippocampal membranes for 180 min with dihydroergosine (2 nmol dm(-3)) completely prevented the binding of (H-3)8-OH-DPAT (2 nmol dm(-3)). The data suggest that dihydroergosine is approximately as potent a ligand as 8-OH-DPAT for the hippocampal 5-HT1A receptors from human and rat brains, although their kinetics of association and dissociation are apparently different.
||ergot alkaloid; 5-HT1A receptors; (H-3)8-OH-DPAT binding; hippocampus; human brain; rat brain; antidepressant dihydroergosine; serotonin receptors; messenger-rna; sites; subtypes; radioligand; cortex; 8-hydroxy-2-(di-normal-propylamino)tetralin; selectivity; anxiety
||NATURAL SCIENCES > Chemistry
||Division of Molecular Medicine
|Project title||Project leader||Project code||Project type|
|Neurofarmakologija GABA i 5-HT sustava||Danka Peričić||00980001||MZOS|
|Neurofarmakologija serotoninskog sustava||Dorotea Muck-Šeler||0098088||MZOS|
|Neurotransmitori u stresu i regulacija GABA receptora in vitro||Danka Peričić||0098103||MZOS|
Dorotea Mueck Šeler
||19 Nov 2013 11:54
||28 Feb 2014 14:29
Actions (login required)