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Cholinesterase activity in the tissues of bivalves Noah's ark shell (Arca noae) and warty venus (Venus verrucosa): Characterisation and in vitro sensitivity to organophosphorous pesticide trichlorfon

Perić, Lorena; Ribarić, Luka; Nerlović, Vedrana (2013) Cholinesterase activity in the tissues of bivalves Noah's ark shell (Arca noae) and warty venus (Venus verrucosa): Characterisation and in vitro sensitivity to organophosphorous pesticide trichlorfon. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 165 (4). pp. 243-249. ISSN 1096-4959

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Abstract

Cholinesterase (ChE) activity was investigated in gills and adductor muscle of two bivalve species: Arca noae and Venus verrucosa. The properties of ChEs were investigated using acetylcholine iodide (ASCh), butyrylcholine iodide (BSCh) and propionylcholin iodide (PrSCh) as substrates and eserine, BW254c51 and iso-OMPA as specific inhibitors. The highest level of ChE activity in crude tissue extracts was detected with PrSCh followed by ASCh, while values obtained with BSCh were apparently low, except in A. noae adductor muscle. The enzyme activity in A. noae gills and V. verrucosa gills and adductor muscle was significantly inhibited by BW254c51, but not with iso-OMPA. ChE activity in adductor muscle of A. noae was significantly reduced by both diagnostic inhibitors. The effect of organophosphorous pesticide trichlorfon on ChE activity was investigated in vitro in both species as well as in the gills of mussels Mytilus galloprovincialis. The highest sensitivity of ChE to trichlorfon was observed in A. noae gills and adductor muscle (IC50 1.6×10-7 M and 1.1×10-7 M, respectively), followed by M. galloprovincialis gills (IC50 1.0×10-6 M) and V. verrucosa gills and adductor muscle (IC50 1.7×10-5 M and 0.9x10-5 M, respectively). The results of this study suggest the potential of ChE activity measurement in the tissues of A. noae as effective biomarker of OP exposure in marine environment

Item Type: Article
Uncontrolled Keywords: Arca noae; biomarker; bivalves; cholinesterase activity; trichlorfon; Venus verrucosa
Subjects: NATURAL SCIENCES > Biology
NATURAL SCIENCES > Interdisciplinary Natural Sciences > Marine Science
Divisions: Center for Marine Research
Projects:
Project titleProject leaderProject codeProject type
Ekotoksični učinci onečišćenja na morske organizme[104652] Nevenka Bihari098-0982705-2725MZOS
Depositing User: Lorena Perić
Date Deposited: 15 Nov 2013 11:45
Last Modified: 26 Mar 2015 09:58
URI: http://fulir.irb.hr/id/eprint/945
DOI: 10.1016/j.cbpb.2013.05.002

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