Miloš, Tina; Rojo, David; Nedić Erjavec, Gordana; Konjevod, Marcela; Tudor, Lucija; Vuić, Barbara; Švob Štrac, Dubravka; Uzun, Suzana; Mimica, Ninoslav; Kozumplik, Oliver; Barbas, Coral; Žarković, Neven; Pivac, Nela; Nikolac Perković, Matea (2023) Metabolic profiling of Alzheimer's disease: Untargeted metabolomics analysis of plasma samples. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 127 . ISSN 0278-5846
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Abstract
Alzheimer's disease (AD) is often not recognized or is diagnosed very late, which significantly reduces the effectiveness of available pharmacological treatments. Metabolomic analyzes have great potential for improving existing knowledge about the pathogenesis and etiology of AD and represent a novel approach towards discovering biomarkers that could be used for diagnosis, prognosis, and therapy monitoring. In this study, we applied the untargeted metabolomic approach to investigate the changes in biochemical pathways related to AD pathology. We used gas chromatography and liquid chromatography coupled to mass spectrometry (GC–MS and LC-MS, respectively) to identify metabolites whose levels have changed in subjects with AD diagnosis (N = 40) compared to healthy controls (N = 40) and individuals with mild cognitive impairment (MCI, N = 40). The GC–MS identified significant differences between groups in levels of metabolites belonging to the classes of benzene and substituted derivatives, carboxylic acids and derivatives, fatty acyls, hydroxy acids and derivatives, keto acids and derivatives, and organooxygen compounds. Most of the compounds identified by the LC-MS were various fatty acyls, glycerolipids and glycerophospholipids. All of these compounds were decreased in AD patients and in subjects with MCI compared to healthy controls. The results of the study indicate disturbed metabolism of lipids and amino acids and an imbalance of metabolites involved in energy metabolism in individuals diagnosed with AD, compared to healthy controls and MCI subjects.
| Item Type: | Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Uncontrolled Keywords: | Alzheimer’s disease ; mild cognitive impairment ; metabolomics ; untargeted ; plasma | ||||||||
| Subjects: | BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences | ||||||||
| Divisions: | Division of Molecular Medicine | ||||||||
| Projects: |
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| Depositing User: | Matea Nikolac Perković | ||||||||
| Date Deposited: | 08 Jan 2025 08:37 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/9383 | ||||||||
| DOI: | 10.1016/j.pnpbp.2023.110830 |
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