Stability of the complex between yeast seryl-tRNA synthetase and tRNA(Ser) under different electrophoretic conditions

Gruić-Sovulj, Ita; Rokov-Plavec, Jasmina; Močibob, Marko; Kamenski, Tomislav; Weygand-Đurašević, Ivana (2004) Stability of the complex between yeast seryl-tRNA synthetase and tRNA(Ser) under different electrophoretic conditions. Croatica Chemica Acta, 77 (4). pp. 599-604. ISSN 0011-1643

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Abstract

Noncovalent interactions of yeast homodimeric seryl-tRNA synthetase (SerRS) and cognate tRNA(Ser) were studied by the gel mobility shift assay and zone-interference gel electrophoresis performed under the same binding and electrophoretic conditions. Purified tRNA(Ser) as well as total yeast tRNA were applied as ligands. In the absence of Mg2+ SerRS:(tRNA(Ser))(1) noncovalent complex was detected only by zone-interference gel electrophoresis. K-d values determined in the presence and absence of Mg2+ were in the same range, suggesting that Mg2+ ions mainly influence dissociation-association kinetics of the complex, with a minor contribution to its thermodynamic stability. Comparison of these two assays was shown to be useful in the analysis of thermodynamic and kinetic properties of protein:nucleic acid complexes.

Item Type:

Article

Uncontrolled Keywords:

aminoacyl-tRNA synthetase; gel mobility shift assay; zone-interference gel electrophoresis; SerRS : tRNAs(Ser) noncovalent complexes; Mg2+ influence; aminoacyl-transfer-rna; elongation-factor-tu; escherichia-coli; gel-electrophoresis; thermus-thermophilus; transfer rna(ser); binding; recognition; specificity; magnesium

Subjects:

NATURAL SCIENCES > Chemistry

Divisions:

Division of Molecular Medicine

Projects: